Human p21/CIP1/CDKN1A Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1047V-100UG
Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry.
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Human p21/CIP1/CDKN1A Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human p21/CIP1/CDKN1A in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 195720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p21/CIP1/CDKN1A
Ser2-Pro164
Accession # P38936
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line either treated with 1 μM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Alexa Fluor® 405‑conjugated Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # IC1047V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: p21/CIP1/CDKN1A

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Entrez Gene IDs
1026 (Human); 12575 (Mouse); 114851 (Rat)
Alternate Names
CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1

Product Datasheets

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