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Product Details
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Species ReactivityHuman
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SpecificityDetects human p21/CIP1/CDKN1A in direct ELISAs and Western blots.
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SourceMonoclonal Mouse IgG2A Clone # 195720
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PurificationProtein A or G purified from hybridoma culture supernatant
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ImmunogenE. coli-derived recombinant human p21/CIP1/CDKN1A
Ser2-Pro164
Accession # P38936 -
FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
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LabelAlexa Fluor 405
Product Datasheets
Certificate of Analysis Lookup
Certificate of Analysis Lookup
Certificate of Analysis Request Form
Applications
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Recommended
ConcentrationSample
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Intracellular Staining by Flow Cytometry0.25-1 µg/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application.
General Protocols
are available in the Technical Information section on our website.
Data Example
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Intracellular Staining by Flow Cytometry
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Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line either treated with 1 μM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Alexa Fluor® 405‑conjugated Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # IC1047V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Preparation and Storage
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ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
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Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: p21/CIP1/CDKN1A
p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.
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Long Name:p21 Cyclin Dependent Kinase 4 Inhibitor 1A
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Entrez Gene IDs:1026 (Human); 12575 (Mouse); 114851 (Rat)
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Alternate Names:CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1
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