Human p21/CIP1/CDKN1A Alexa Fluor® 405-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human p21/CIP1/CDKN1A in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 195720
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human p21/CIP1/CDKN1A
    Ser2-Pro164
    Accession # P38936
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line either treated with 1 μM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Alexa Fluor® 405‑conjugated Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # IC1047V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: p21/CIP1/CDKN1A

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

  • Long Name:
    p21 Cyclin Dependent Kinase 4 Inhibitor 1A
  • Entrez Gene IDs:
    1026 (Human); 12575 (Mouse); 114851 (Rat)
  • Alternate Names:
    CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1
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