Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody Summary
Accession # P38936
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line either treated with 1 µM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Alexa Fluor® 700-conjugated Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # IC1047N). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human p21/CIP1/CDKN1A Alexa Fluor® 700-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image