PAK1 (p21-activaed kinase 1; also alpha -PAK and p65-PAK) is both a cytoplasmic and nuclear 58-60 kDa member of PAK group I, STE20 subfamily, STE Ser/Thr protein kinase family of molecules. It is widely expressed, and is upregulated in numerous cancers. PAK1 has multiple activities. It is responsible for regulating focal adhesions during motility. At the front edge of cells, PAK1 promotes focal adhesion formation by phosphorylating PIX, while at the back edge, it directs focal adhesion disassembly. PAK1 also impacts microtubule formation. Microtubules are composed of heterodimeric subunits containing an alpha - and beta -tubulin polypeptide. The availability of alpha -tubulin for microtubule formation is based on the ability of PAK1 to phosphorylate a alpha -tubulin chaperone, tubulin cofactor B. PAK1 is activated following binding to active GTPases which induce PAK autophosphorylation. When active, PAK1 is a monomer; when inactive, it reportedly homodimerizes. Human PAK1 is 545 amino acids (aa) in length. It contains one GTPase binding domain (aa 75-105) plus a protein kinase catalytic region (aa 250-521). There are five SH3-binding motifs. Acetylation occurs on Lys256, and there are at least 14 potential phosphorylation sites, seven of which are known to be utilized. There is one splice variant that shows a 35 aa substitution for aa 518-545. Over aa 128-242, human PAK1 shares 96% aa sequence identity with mouse PAK1.
Human PAK1 Alexa Fluor® 532‑conjugated Antibody
R&D Systems | Catalog # AF7495X
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Leu128-Gln242
Accession # Q13153
Specificity
Clonality
Host
Isotype
Applications for Human PAK1 Alexa Fluor® 532‑conjugated Antibody
Immunocytochemistry
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: PAK1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PAK1 Products
Product Documents for Human PAK1 Alexa Fluor® 532‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human PAK1 Alexa Fluor® 532‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars