Human PAK2 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6849U Datasheet / COA / SDS

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FAB6849U-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PAK2 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PAK2 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) PAK1 and no cross-reactivity with rhPAK1B, 3, 4, 6, or 7 is observed.

Source

Monoclonal Mouse IgG3 Clone # 677411

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PAK2
Leu127-Gln221
Accession # Q13177

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PAK2

PAK2 (p21-activated kinase-2) is a widely expressed 58-62 kDa cytoplasmic ser/thr kinase of the PAK family, with substrates including ribosomal S6 and histone H4 proteins. The 524 amino acid (aa) human PAK2 contains phosphorylation, acetylation (K38, K128) and myristoylation (G213) sites, a CRIB (cdc42/Rac interactive binding) domain (aa 74-87), a caspase-3 cleavage site after aa 212, and a kinase domain (aa 249-499). Full-length PAK2 stimulates cell growth and motility, while the caspase cleaved PAK2 kinase domain (34 kDa) is involved in cell death. Within the region used as an immunogen, human PAK2 shares 87% aa identity with mouse and rat PAK2.

Long Name

p21-activated Kinase 2

Entrez Gene IDs

5062 (Human); 224105 (Mouse); 29432 (Rat)

Alternate Names

EC 2.7.11; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; PAK2; PAK-2; PAK65; PAK65EC 2.7.11.1; PAKgamma; S6/H4 Kinase; serine/threonine-protein kinase PAK 2