Detection of Human, Mouse, and Rat PAK2 by Western Blot.
Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAK2 Monoclonal Antibody (Catalog # MAB6849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PAK2 (p21-activated kinase-2) is a widely expressed 58-62 kDa cytoplasmic ser/thr kinase of the PAK family, with substrates including ribosomal S6 and histone H4 proteins. The 524 amino acid (aa) human PAK2 contains phosphorylation, acetylation (K38, K128) and myristoylation (G213) sites, a CRIB (cdc42/Rac interactive binding) domain (aa 74-87), a caspase-3 cleavage site after aa 212, and a kinase domain (aa 249-499). Full-length PAK2 stimulates cell growth and motility, while the caspase cleaved PAK2 kinase domain (34 kDa) is involved in cell death. Within the region used as an immunogen, human PAK2 shares 87% aa identity with mouse and rat PAK2.
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