Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry.
Y‑79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB5387G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PEDF R/PNPLA2
PEDF R (Pigment Epithelium-derived Factor Receptor), also known as PNPLA2, ATGL, TTS2.2, iPLA2 zeta (Calcium-independent Phospholipase A2 zeta) and Desnutrin, is a 82-86 kDa type II transmembrane protein that is a member of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue (both white and brown fat cells), where it the catalyzes formation of diacyglycerol from triglyceride, the transfer of an acyl (CoA-fatty acid) derivative to acyl-glycerol, and releases arachidonic acid from intracellular lipid deposits. It has also been found on retinal pigment epithelium, endothelial cells, hepatocytes, cerebellar granule cells, macrophages, mast cells, ovarian granulosa cells, retinal ganglia neurons and skeletal muscle cells. PEDF R is directly activated by PEDF, and indirectly by beta 3-AR which promotes CGI-58 association. Human PEDF R shares 85% amino acid (aa) sequence identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
Have you used Human PEDF R/PNPLA2 Alexa Fluor® 488-conjugated Antibody?
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