Human PEDF R/PNPLA2 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human PEDF R/PNPLA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 494702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PEDF R/PNPLA2
    Accession # Q96AD5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry. Y‑79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB5387G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PEDF R/PNPLA2

PEDF R (Pigment Epithelium-derived Factor Receptor), also known as PNPLA2, ATGL, TTS2.2, iPLA2 zeta (Calcium-independent Phospholipase A2 zeta) and Desnutrin, is a 82-86 kDa type II transmembrane protein that is a member of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue (both white and brown fat cells), where it the catalyzes formation of diacyglycerol from triglyceride, the transfer of an acyl (CoA-fatty acid) derivative to acyl-glycerol, and releases arachidonic acid from intracellular lipid deposits. It has also been found on retinal pigment epithelium, endothelial cells, hepatocytes, cerebellar granule cells, macrophages, mast cells, ovarian granulosa cells, retinal ganglia neurons and skeletal muscle cells. PEDF R is directly activated by PEDF, and indirectly by  beta 3-AR which promotes CGI-58 association. Human PEDF R shares 85% amino acid (aa) sequence identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.

  • Long Name:
    Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
  • Entrez Gene IDs:
    57104 (Human); 66853 (Mouse); 361676 (Rat)
  • Alternate Names:
    Adipose triglyceride lipase; ATGL; ATGL1110001C14Rik; Calcium-independent phospholipase A2; Desnutrin; EC; FP17548; IPLA2-zeta; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2-like; PEDFR; PEDF-R; Pigment epithelium-derived factor; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2; TTS2DKFZp667M109
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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