Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

(12 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    5 hours
  • Sample Type & Volume Required Per Well
    Serum-free Cell Culture Supernates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL)
  • Sensitivity
    0.116 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
  • Specificity
    Natural and recombinant human PTX3
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum-free Cell Culture Supern, EDTA Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.010.280.620.170.390.6


The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 83-106
Serum-free Cell Culture Media (n=4) 101 95-108
To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
    • Long Name
      Tumor Necrosis Factor Stimulated Gene 14
    • Entrez Gene IDs
      5806 (Human); 19288 (Mouse);
    • Alternate Names
      alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 20 µL Pretreated Standard, Control, or Sample
    6.   Add 20 µL of pretreated Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 12
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    Sample Type
    1. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
      Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
      PLoS ONE, 2015;10(6):e0130166.
      Species: Human
      Sample Type: Serum
    2. Chemerin and adiponectin contribute reciprocally to metabolic syndrome.
      Authors: Chu SH, Lee MK, Ahn KY, Im JA, Park MS, Lee DC, Jeon JY, Lee JW
      PLoS ONE, 2012;7(4):e34710.
      Species: Human
      Sample Type: Plasma
    3. A multi-analyte assay for the non-invasive detection of bladder cancer.
      Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
      PLoS ONE, 2012;7(10):e47469.
      Species: Human
      Sample Type: Urine
    4. Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
      Authors: Outinen TK, Kuparinen T, Jylhava J, Leppanen S, Mustonen J, Makela S, Porsti I, Syrjanen J, Vaheri A, Hurme M
      PLoS ONE, 2012;7(2):e31455.
      Species: Human
      Sample Type: Plasma
    5. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
      Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
      PLoS ONE, 2012;7(4):e36056.
      Species: Human
      Sample Type: Serum
    6. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
      Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
      PLoS ONE, 2012;7(4):e34965.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
      Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
      Clin. Cancer Res., 2011;17(8):2395-2399.
      Species: Human
      Sample Type: Serum
    8. High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study.
      Authors: Huttunen R, Hurme M, Aittoniemi J, Huhtala H, Vuento R, Laine J, Jylhava J, Syrjanen J
      PLoS ONE, 2011;6(3):e17653.
      Species: Human
      Sample Type: Plasma
    9. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
      Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
      Cell, 2011;145(6):926-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey.
      Authors: Jylhava J, Haarala A, Kahonen M, Lehtimaki T, Jula A, Moilanen L, Kesaniemi YA, Nieminen MS, Hurme M
      Clin. Exp. Immunol., 2011;164(2):211-7.
      Species: Human
      Sample Type: Plasma
    11. Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women.
      Authors: May L, Kuningas M, van Bodegom D
      Biol. Reprod., 2010;82(2):299-304.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility?
      Authors: Miyaki A, Maeda S, Yoshizawa M
      J. Atheroscler. Thromb., 2010;17(3):278-84.
      Species: Human
      Sample Type: Plasma
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 703 for Human Pentraxin 3

    Ctrl QC151
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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