Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Human Pentraxin 3/TSG-14 ELISA Standard Curve
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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary

Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), EDTA Plasma (20 uL), Saliva (20 uL)
Sensitivity
0.026 ng/mL
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human Pentraxin 3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Pentraxin 3/TSG-14 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human Pentraxin 3 in serum-free cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human Pentraxin 3 and antibodies raised against the recombinant protein. Natural human Pentraxin 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human
Pentraxin 3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 2.12 6.03 11.6 2.05 5.86 11.4
Standard Deviation 0.038 0.158 0.14 0.141 0.383 0.5
CV% 1.8 2.6 1.2 6.9 6.5 4.4

Recovery

The recovery of human Pentraxin 3 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 85-108
Saliva (n=4) 89 79-99
Serum-free Cell Culture Media (n=2) 109 100-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Pentraxin 3/TSG-14 ELISA Linearity

Data Examples

Human Pentraxin 3/TSG-14 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Citations for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Class II obese and healthy pregnant controls exhibit indistinguishable pro- and anti-inflammatory immune responses to Caesarian section
    Authors: C Graham, M Thorleifso, WP Stefura, DJ Funk, KT HayGlass
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD
    Authors: SJ Thulborn, M Dilpazir, K Haldar, V Mistry, CE Brightling, MR Barer, M Bafadhel
    Int J Chron Obstruct Pulmon Dis, 2017;12(0):1199-1205.
    Species: Human
    Sample Types: Sputum
  3. Diagnostic value of Pentraxin-3 in patients with sepsis and septic shock in accordance with latest sepsis-3 definitions
    Authors: S Hamed, M Behnes, D Pauly, D Lepiorz, M Barre, T Becher, S Lang, I Akin, M Borggrefe, T Bertsch, U Hoffmann
    BMC Infect. Dis., 2017;17(1):554.
    Species: Human
    Sample Types: Plasma
  4. The diagnostic value of serum pentraxin 3 levels in pulmonary contusion
    Authors: Suha Turkmen
    Am J Emerg Med, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Pseudomonas aeruginosa GroEL stimulates production of PTX3 by activating the NF-?B pathway and simultaneously down-regulating microRNA-9
    Infect. Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Sputum pentraxin 3 as a candidate to assess airway inflammation and remodeling in childhood asthma
    Authors: Min Jung Kim
    Medicine (Baltimore), 2016;95(51):e5677.
    Species: Human
    Sample Types: Sputum
  7. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells
    Sci Rep, 2016;6(0):37128.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
    Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
    PLoS ONE, 2015;10(6):e0130166.
    Species: Human
    Sample Types: Serum
  9. Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
    Authors: Outinen TK, Kuparinen T, Jylhava J, Leppanen S, Mustonen J, Makela S, Porsti I, Syrjanen J, Vaheri A, Hurme M
    PLoS ONE, 2012;7(2):e31455.
    Species: Human
    Sample Types: Plasma
  10. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Chemerin and adiponectin contribute reciprocally to metabolic syndrome.
    Authors: Chu SH, Lee MK, Ahn KY, Im JA, Park MS, Lee DC, Jeon JY, Lee JW
    PLoS ONE, 2012;7(4):e34710.
    Species: Human
    Sample Types: Plasma
  12. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
    Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
    PLoS ONE, 2012;7(4):e36056.
    Species: Human
    Sample Types: Serum
  13. A multi-analyte assay for the non-invasive detection of bladder cancer.
    Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
    PLoS ONE, 2012;7(10):e47469.
    Species: Human
    Sample Types: Urine
  14. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
    Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
    Clin. Cancer Res., 2011;17(8):2395-2399.
    Species: Human
    Sample Types: Serum
  15. Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey.
    Authors: Jylhava J, Haarala A, Kahonen M, Lehtimaki T, Jula A, Moilanen L, Kesaniemi YA, Nieminen MS, Hurme M
    Clin. Exp. Immunol., 2011;164(2):211-7.
    Species: Human
    Sample Types: Plasma
  16. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
    Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
    Cell, 2011;145(6):926-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study.
    Authors: Huttunen R, Hurme M, Aittoniemi J, Huhtala H, Vuento R, Laine J, Jylhava J, Syrjanen J
    PLoS ONE, 2011;6(3):e17653.
    Species: Human
    Sample Types: Plasma
  18. Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility?
    Authors: Miyaki A, Maeda S, Yoshizawa M
    J. Atheroscler. Thromb., 2010;17(3):278-84.
    Species: Human
    Sample Types: Plasma
  19. Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women.
    Authors: May L, Kuningas M, van Bodegom D
    Biol. Reprod., 2010;82(2):299-304.
    Species: Human
    Sample Types: Cell Culture Supernates

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