Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DPTX30B
Control Products Available
Human Pentraxin 3/TSG-14 ELISA Standard Curve
2 Images
Product Details
Citations (22)
FAQs
Supplemental Products
Reviews

Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary

Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), EDTA Plasma (20 uL), Saliva (20 uL)
Sensitivity
0.026 ng/mL
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human Pentraxin 3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Pentraxin 3/TSG-14 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human Pentraxin 3 in serum-free cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human Pentraxin 3 and antibodies raised against the recombinant protein. Natural human Pentraxin 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human
Pentraxin 3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 2.12 6.03 11.6 2.05 5.86 11.4
Standard Deviation 0.038 0.158 0.14 0.141 0.383 0.5
CV% 1.8 2.6 1.2 6.9 6.5 4.4

Recovery

The recovery of human Pentraxin 3 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 85-108
Saliva (n=4) 89 79-99
Serum-free Cell Culture Media (n=2) 109 100-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Pentraxin 3/TSG-14 ELISA Linearity

Data Examples

Human Pentraxin 3/TSG-14 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Citations for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Long Pentraxin 3 as a Broader Biomarker for Multiple Risk Factors in End-Stage Renal Disease: Association with All-Cause Mortality
    Authors: MJ Valente, S Rocha, S Coimbra, C Catarino, P Rocha-Pere, E Bronze-da-, JG Oliveira, J Madureira, JC Fernandes, M do Sameiro, V Miranda, L Belo, A Santos-Sil
    Mediators Inflamm., 2019;2019(0):3295725.
    Species: Human
    Sample Types: Plasma
  2. Inevitable isolation and the change of stress markers in hemodialysis patients during the 2015 MERS-CoV outbreak in Korea
    Authors: YG Kim, H Moon, SY Kim, YH Lee, DW Jeong, K Kim, JY Moon, YK Lee, A Cho, HS Lee, HC Park, SH Lee
    Sci Rep, 2019;9(1):5676.
    Species: Human
    Sample Types: Serum
  3. Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis
    Authors: Y Takahashi, R Watanabe, Y Sato, N Ozawa, M Kojima, K Watanabe-K, R Shirai, K Sato, T Hirano, T Watanabe
    Metab. Clin. Exp., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Class II obese and healthy pregnant controls exhibit indistinguishable pro- and anti-inflammatory immune responses to Caesarian section
    Authors: C Graham, M Thorleifso, WP Stefura, DJ Funk, KT HayGlass
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Investigating the role of pentraxin 3 as a biomarker for bacterial infection in subjects with COPD
    Authors: SJ Thulborn, M Dilpazir, K Haldar, V Mistry, CE Brightling, MR Barer, M Bafadhel
    Int J Chron Obstruct Pulmon Dis, 2017;12(0):1199-1205.
    Species: Human
    Sample Types: Sputum
  6. Diagnostic value of Pentraxin-3 in patients with sepsis and septic shock in accordance with latest sepsis-3 definitions
    Authors: S Hamed, M Behnes, D Pauly, D Lepiorz, M Barre, T Becher, S Lang, I Akin, M Borggrefe, T Bertsch, U Hoffmann
    BMC Infect. Dis., 2017;17(1):554.
    Species: Human
    Sample Types: Plasma
  7. The diagnostic value of serum pentraxin 3 levels in pulmonary contusion
    Authors: Suha Turkmen
    Am J Emerg Med, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Sputum pentraxin 3 as a candidate to assess airway inflammation and remodeling in childhood asthma
    Authors: Min Jung Kim
    Medicine (Baltimore), 2016;95(51):e5677.
    Species: Human
    Sample Types: Sputum
  9. Pseudomonas aeruginosa GroEL stimulates production of PTX3 by activating the NF-?B pathway and simultaneously down-regulating microRNA-9
    Infect. Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells
    Sci Rep, 2016;6(0):37128.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
    Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
    PLoS ONE, 2015;10(6):e0130166.
    Species: Human
    Sample Types: Serum
  12. Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
    Authors: Outinen TK, Kuparinen T, Jylhava J, Leppanen S, Mustonen J, Makela S, Porsti I, Syrjanen J, Vaheri A, Hurme M
    PLoS ONE, 2012;7(2):e31455.
    Species: Human
    Sample Types: Plasma
  13. Chemerin and adiponectin contribute reciprocally to metabolic syndrome.
    Authors: Chu SH, Lee MK, Ahn KY, Im JA, Park MS, Lee DC, Jeon JY, Lee JW
    PLoS ONE, 2012;7(4):e34710.
    Species: Human
    Sample Types: Plasma
  14. A multi-analyte assay for the non-invasive detection of bladder cancer.
    Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
    PLoS ONE, 2012;7(10):e47469.
    Species: Human
    Sample Types: Urine
  15. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
    Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
    PLoS ONE, 2012;7(4):e36056.
    Species: Human
    Sample Types: Serum
  16. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
    Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
    Cell, 2011;145(6):926-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Pentraxin 3 (PTX3) is associated with cardiovascular risk factors: the Health 2000 Survey.
    Authors: Jylhava J, Haarala A, Kahonen M, Lehtimaki T, Jula A, Moilanen L, Kesaniemi YA, Nieminen MS, Hurme M
    Clin. Exp. Immunol., 2011;164(2):211-7.
    Species: Human
    Sample Types: Plasma
  19. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
    Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
    Clin. Cancer Res., 2011;17(8):2395-2399.
    Species: Human
    Sample Types: Serum
  20. High Plasma Level of Long Pentraxin 3 (PTX3) Is Associated with Fatal Disease in Bacteremic Patients: A Prospective Cohort Study.
    Authors: Huttunen R, Hurme M, Aittoniemi J, Huhtala H, Vuento R, Laine J, Jylhava J, Syrjanen J
    PLoS ONE, 2011;6(3):e17653.
    Species: Human
    Sample Types: Plasma
  21. Is pentraxin 3 involved in obesity-induced decrease in arterial distensibility?
    Authors: Miyaki A, Maeda S, Yoshizawa M
    J. Atheroscler. Thromb., 2010;17(3):278-84.
    Species: Human
    Sample Types: Plasma
  22. Genetic variation in pentraxin (PTX) 3 gene associates with PTX3 production and fertility in women.
    Authors: May L, Kuningas M, van Bodegom D
    Biol. Reprod., 2010;82(2):299-304.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

ELISA Controls

Supplemental ELISA Products

Reviews for Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human Pentraxin 3/TSG-14 Quantikine ELISA Kit and earn rewards!

Have you used Human Pentraxin 3/TSG-14 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review