Human Phospho-STAT2 (Y689) Alexa Fluor® 488‑conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8627G-025
Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry.
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Human Phospho-STAT2 (Y689) Alexa Fluor® 488‑conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human phospho-STAT2 (Y689) in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1021D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human STAT2 Y689 site
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes was stained with Rabbit Anti-Human STAT2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8627G, filled histogram) or isotype control antibody (Catalog # IC105G, not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT2

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name
Signal Transducer and Activator of Transcription 2
Entrez Gene IDs
6773 (Human); 20847 (Mouse)
Alternate Names
interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2

Product Datasheets

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