Human PIAS2 Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PIAS2

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-beta ) is a 45‑70 kDa member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK‑1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa 11‑45), a zinc-finger region (aa 331‑408), a SUMO-binding motif (aa 467‑472) and an NLS (aa 484‑492). There are multiple splice variants. PIAS2x-beta /ARIP3 shows a 22 aa substitution for aa 551‑621, while PIAS-NY shows a 12 aa substitution for aa 1‑8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504‑621, and an alternate start site at Met10. Over aa 1‑550, human PIAS2 shares 98% aa identity with mouse PIAS2.

Product Datasheets

Related Research Areas

• Negative Regulators of the Jak/STAT Pathway
• Stem Cell Transcription Factor Regulators
• Ubiquitin-like Modifiers (UBLs) and Regulators