Human PILR-alpha Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB64841R-100
Detection of PILR-alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry
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Human PILR-alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PILR-alpha in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2175D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR-alpha with a C-terminal 6-His tag
Gln20-Thr196
Accession # Q9UKJ1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of PILR-alpha R antibody in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP antibody by Flow Cytometry View Larger

Detection of PILR-alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human PILR-alpha or (B) irrelevant transfectants and eGFP was stained with Alexa Fluor® 647-conjugated Rabbit Anti-Human PILR-alpha Monoclonal Antibody (Catalog # FAB64841R). Quadrant markers were set based on control antibody staining (Catalog # IC1051R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PILR-alpha

Paired immunoglobulin-like type 2 receptor alpha (PILRa; also inhibitory receptor PILR-alpha) are 44-50 kDa paired receptors that consist of highly related activating and inhibitory receptors, and are widely involved in the regulation of the immune system. PILR-alpha is thought to act as a cellular signaling inhibitory receptor by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2 via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Human PILR-alpha is synthesized as a 303 amino acid (aa) precursor that contains a 19 aa signal sequence, a 178 aa extracellular domain (ECD), a 21 aa transmembrane segment, and an 85 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and one potential site for N-linked glycosylation. The cytoplasmic domain contains two ITIM motifs (aa 267-272 and 296-301). Alternate splicing generates multiple shorter isoforms. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Mature human PILR-alpha is 45% aa identical to mature mouse PILR-alpha. PILR-alpha is predominantly detected in hemopoietic tissues and is expressed in monocytes, macrophages, and granulocytes, but not lymphocytes. It is also strongly expressed by dendritic cells. PILR-alpha interacts with herpes simplex 1 glycoprotein B and functions as an entry coreceptor for this virus. 

Long Name
Paired-Ig-likeType 2 Receptor alpha
Entrez Gene IDs
29992 (Human); 231805 (Mouse); 288568 (Rat)
Alternate Names
Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha

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