Human PILR-alpha APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6484A
Detection of PILR‑ alpha  in Human Blood Granulocytes by Flow Cytometry.
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Reviews (10)

Human PILR-alpha APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PILR-alpha in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
Gln20-Thr196
Accession # Q9UKJ1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of PILR-a antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of PILR‑ alpha in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6484A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name
Paired-Ig-likeType 2 Receptor alpha
Entrez Gene IDs
29992 (Human); 231805 (Mouse); 288568 (Rat)
Alternate Names
Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Human PILR-alpha APC-conjugated Antibody

Average Rating: 5 (Based on 10 Reviews)

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Human PILR-alpha APC-conjugated Antibody
By Anonymous on 04/12/2018
Application: Flow Cytometry Sample Tested: B16-F10 mouse melanoma cell line Species: Mouse

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 03/30/2018
Application: ELISA Sample Tested: B16-F10 mouse melanoma cell line Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 03/11/2018
Application: Flow Cytometry Sample Tested: A375 human melanoma cell line Species: Human

Human PILR-alpha APC-conjugated Antibody
By Sagar Kale on 11/27/2017
Application: Flow Cytometry Sample Tested: Natural killer cells Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 11/27/2017
Application: Flow Cytometry Sample Tested: Human NK cells Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 11/18/2017
Application: Flow Cytometry Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 11/10/2017
Application: Flow Cytometry Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 10/02/2017
Application: Flow Cytometry Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 09/25/2017
Application: Flow Cytometry Sample Tested: 4T1 mouse breast cancer cell line Species: Human

Human PILR-alpha APC-conjugated Antibody
By Anonymous on 09/23/2017
Application: Flow Cytometry Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human