Human PILR-alpha APC-conjugated Antibody

(10 Reviews)
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human PILR-alpha in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
    Gln20-Thr196
    Accession # Q9UKJ1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of PILR‑ alpha in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR‑ alpha APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6484A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

  • Long Name:
    Paired-Ig-likeType 2 Receptor alpha
  • Entrez Gene IDs:
    29992 (Human); 231805 (Mouse); 288568 (Rat)
  • Alternate Names:
    Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG APC-conjugated Antibody

Ctrl IC016A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 10 reviews)

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Read what people are saying who have used Human PILR-alpha APC-conjugated Antibody.

We have 10 reviews tested in 2 species: Human, Mouse.
We have 10 reviews tested in 2 applications: Flow Cytometry, ELISA.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Mouse Anonymous 04/12/2018
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Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
  Excellent
 ELISA Human Anonymous 03/30/2018
Show

Summary

Application ELISA
Sample Tested B16-F10 mouse melanoma cell line
Species Human
  Excellent
 Flow Human Anonymous 03/11/2018
Show

Summary

Application Flow Cytometry
Sample Tested A375 human melanoma cell line
Species Human
  Excellent
 Flow Human Anonymous 11/27/2017
Show

Summary

Application Flow Cytometry
Sample Tested Natural killer cells
Species Human
  Excellent
 Flow Human Anonymous 11/27/2017
Show

Summary

Application Flow Cytometry
Sample Tested Human NK cells
Species Human
  Excellent
 Flow Human Anonymous 11/18/2017
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Summary

Application Flow Cytometry
Sample Tested Peripheral blood mononuclear cells (PBMCs)
Species Human
  Excellent
 Flow Human Anonymous 11/10/2017
Show

Summary

Application Flow Cytometry
Sample Tested Blood mononuclear cells (PBMCs)
Species Human
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A Array FAB6484A
Enlarge
Excellent
 Flow Human Anonymous 10/02/2017
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Image Details
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A
Flow Cytometry: Human PILR-alpha APC-conjugated Antibody [FAB6484A].

Summary

Application Flow Cytometry
Sample Tested Jurkat human acute T cell leukemia cell line
Species Human
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A Array FAB6484A
Enlarge
Excellent
 Flow Human Anonymous 09/25/2017
Show
Image Details
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A
Flow Cytometry: Human PILR-alpha APC-conjugated Antibody [FAB6484A].

Summary

Application Flow Cytometry
Sample Tested 4T1 mouse breast cancer cell line
Species Human
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A Array FAB6484A
Enlarge
Excellent
 Flow Human Anonymous 09/23/2017
Show
Image Details
Flow Cytometry Human PILR-alpha  APC-conjugated Antibody FAB6484A
Flow Cytometry: Human PILR-alpha APC-conjugated Antibody [FAB6484A].

Summary

Application Flow Cytometry
Sample Tested B cell enriched peripheral blood mononuclear cells (PBMC)
Species Human

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