Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Sheep IgG
View all PILR-alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
Gln20-Thr196
Accession # Q9UKJ1

Specificity

Detects human PILR-alpha in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PILR‑ alpha APC‑conjugated Antibody

Detection of PILR-a antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of PILR‑ alpha in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6484A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PILR‑ alpha APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes

Reviewed Applications

Read 10 reviews rated 5 using FAB6484A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name

Paired-Ig-likeType 2 Receptor alpha

Alternate Names

FDF03, PILRA, PILRalpha

Entrez Gene IDs

29992 (Human); 231805 (Mouse); 288568 (Rat)

Gene Symbol

PILRA

UniProt

Q9UKJ1 (Human)

Additional PILR-alpha Products

Product Documents for Human PILR‑ alpha APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PILR‑ alpha APC‑conjugated Antibody

For research use only

Customer Reviews for Human PILR‑ alpha APC‑conjugated Antibody (10)

5 out of 5
10 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human PILR‑ alpha APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 10 reviews Showing All
Filter By:
Clear Filters X
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 04/12/2018
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Human
    Verified Customer | Posted 03/30/2018
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: A375 human melanoma cell line
    Species: Human
    Verified Customer | Posted 03/11/2018
  • Human PILR-alpha APC-conjugated Antibody
    Name: Sagar Kale
    Application: Flow Cytometry
    Sample Tested: Natural killer cells
    Species: Human
    Verified Customer | Posted 11/27/2017
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human NK cells
    Species: Human
    Verified Customer | Posted 11/27/2017
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 11/18/2017
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 11/10/2017
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 10/02/2017
    Human PILR‑ alpha APC‑conjugated Antibody FAB6484A
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Human
    Verified Customer | Posted 09/25/2017
    Human PILR‑ alpha APC‑conjugated Antibody FAB6484A
  • Human PILR-alpha APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC)
    Species: Human
    Verified Customer | Posted 09/23/2017
    Human PILR‑ alpha APC‑conjugated Antibody FAB6484A

There are no reviews that match your criteria.

Showing  1 - 5 of 10 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies