Sheep IgG APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC016A

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Detection of Sheep IgG Isotype Control by Flow Cytometry
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Product Details
Citations (2)
FAQs
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Reviews (1)

Sheep IgG APC-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) sheep.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Sheep IgG Control by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or Sheep IgG APC-conjugated Isotype Control Antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Sheep IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Conditionally pathogenic genetic variants of a hematopoietic disease-suppressing enhancer
    Authors: AA Soukup, DR Matson, P Liu, KD Johnson, EH Bresnick
    Science Advances, 2021;7(50):eabk3521.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
    Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
    Eur J Immunol, 2013;43(8):2138-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control

FAQs

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Reviews for Sheep IgG APC-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

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Sheep IgG APC-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Cytometry Sample Tested: Adult splenocytes Species: Mouse

Isotype control