Sheep IgG APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC016A
Detection of Sheep IgGControl by Flow Cytometry
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Sheep IgG APC-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) sheep.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Sheep IgGControl by Flow Cytometry HepG2human hepatocellular carcinoma cell line was stained with SheepAnti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC5868A,filled histogram) or Sheep IgG APC-conjugated Isotype Control Antibody(Catalog # IC016A, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer(Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for StainingIntracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citation for Sheep IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
    Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
    Eur J Immunol, 2013;43(8):2138-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype control

FAQs

No product specific FAQs exist for this product, however you may

View all Isotype Control FAQs

Reviews for Sheep IgG APC-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

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Sheep IgG APC-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse

Isotype control