Human Plexin B2 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Plexin B2 in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 537223
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2
    Accession # O15031
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Plexin B2 in Whole Blood Monocytes by Flow Cytometry. Whole blood monocytes were stained with Mouse Anti-Human Plexin B2 APC‑conjugated Monoclonal Antibody (Catalog # FAB53291A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin B2
Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha -chain (aa 20‑1164) and an 80 kDa TM beta ‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.
  • Entrez Gene IDs:
    23654 (Human); 140570 (Mouse); 315217 (Rat)
  • Alternate Names:
    MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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