Human Plexin B3 Alexa Fluor® 488-conjugated Antibody

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FAB4958G-100UG
R&D Systems Antibodies
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Human Plexin B3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin B3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 905805
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Plexin B3
His45-Gln1255
Accession # Q9ULL4
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Plexin B3

Plexin B3 is a type I transmembrane glycoprotein that belongs to the Plexin B subfamily of semaphorin receptors (1, 2). Human Plexin B3 contains a 44 amino acid (aa) signal sequence, a 1211 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other Plexins, such as an LRR (C1) and LRF (C2) domain, and a PDZ motif common to B Plexins (1). The PDZ motif is thought to be involved in RhoA activation and axonal growth cone collapse downstream of semaphorin engagement (3). The human Plexin B3 ECD shares 81%, 81%, 83% and 75% aa identity with mouse, rat, bovine and canine Plexin B3, respectively. It contains a sema domain and a Plexin-Semaphorin-Integrin (PSI) or Met-Related Sequence (MRS) domain, each of which contains a potential proteolytic cleavage site (2). Detection of 160 and 140 kDa species along with the full-length 260 kDa Plexin B3 in homogenates of human neocortex indicates that proteolytic processing may occur in vivo (2). The ECD also contains four glycine/proline-rich IPT/TIG domains, which are immunoglobulin-like domains found in Plexins, transcription factors, and the scatter factor receptors Met and Ron. B Plexins, including Plexin B3, can interact with Met and Ron, activating these receptors upon semaphorin engagement (4, 5). Plexin B3 and its identified ligand, the transmembrane semaphorin Sema5A, are both expressed during differentiation and migration of central nervous system oligodendrocytes (5‑7). However, the developmental functions of Sema5A are likely independent of Plexin B3, which is not significantly expressed prenatally (8). In turn, Plexin B3 may exhibit Sema5A-independent activity, as homophilic interactions of sema domains are reported to stimulate neurite outgrowth of postnatal cerebellar neurons (2).

References
  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Hartwig, C. et al. (2005) BMC Neurosci. 6:53.
  3. Swiercz, J.M. et al. (2002) Neuron 35:51.
  4. Conrotto, P. et al. (2004) Oncogene 23:5131.
  5. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  6. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  7. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  8. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
Entrez Gene IDs
5365 (Human); 140571 (Mouse); 363517 (Rat)
Alternate Names
PLEXB3; Plexin B3; plexin-B3; PLEXR; PLXN6; PLXNB3

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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