Human PlGF Quantikine HS ELISA Kit

R&D Systems | Catalog # HSPG00

R&D Systems
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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

0.326 pg/mL

Assay Range

3.13-200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
View all PlGF ELISA Kits »

Product Summary for Human PlGF Quantikine HS ELISA Kit

The Quantikine HS Human PlGF Immunoassay is a 3.5 hour solid phase
ELISA designed to measure PlGF levels in serum and plasma. It contains
E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human PlGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 27.2 57.4 108 27.3 57.7 108
Standard Deviation 0.72 1.87 2.84 1.65 3.88 7.37
CV% 2.6 3.3 2.6 6.0 6.7 6.8

Recovery for Human PlGF Quantikine HS ELISA Kit

The recovery of PlGF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 98 85-115
Heparin Plasma (n=4) 90 86-97
Serum (n=4) 92 86-97

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PlGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human PlGF ELISA Linearity

Scientific Data Images for Human PlGF Quantikine HS ELISA Kit

Human PlGF ELISA Standard Curve

Human PlGF ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is secreted primarily by villous trophoblasts and decidual cells, with circulating levels increasing during pregnancy but attenuated in preeclampsia. PlGF signals through VEGF R1/Flt‑1 and Neuropilins, while VEGF binds both VEGF R1 and R2 but signals mainly through VEGF R2. PlGF reduces binding of VEGF to VEGF R1 and increases VEGF/VEGF R2‑mediated angiogenesis. It induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing and also contribute to inflammation in active sickle cell disease and atherosclerosis.
 

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

Additional PlGF Products

Product Documents for Human PlGF Quantikine HS ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PlGF Quantikine HS ELISA Kit

For research use only

Citations for Human PlGF Quantikine HS ELISA Kit

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Protocols

View specific protocols for Human PlGF Quantikine HS ELISA Kit (HSPG00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  5.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 6 times.
    8. 50 µL Substrate Solution
  8.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Protect from light. Do not wash the plate.
    9. 50 µL Amplifier Solution
  9.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.

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