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Human PLK1 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3804X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3804X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLK1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLK1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLK1
Ala400-Ser603
Accession # P53350
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PLK1

Polo-like kinase-1 (PLK1) is a serine/threonine kinase that localizes to the mitotic spindle, and is involved in G2 and M phase cell cycle checkpoints. The expression of PLK1 is elevated in many different cancer types. Depletion of PLK1 in mammalian cells results in an inhibition of cell proliferation, decreased viability, and arrest in G2/M phase. PLK1 contains a polo-box domain that binds to phospho-serine and phospho-threonine residues.

Long Name
Polo-like Kinase 1
Entrez Gene IDs
5347 (Human); 18817 (Mouse); 25515 (Rat)
Alternate Names
cell cycle regulated protein kinase; EC 2.7.11; EC 2.7.11.21; PLK; PLK1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase 1polo-like kinase (Drosophila); Serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13

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