Human PLZF APC-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 6318100
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PLZF
    Met1-Gln254
    Accession # Q05516
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry. HL‑60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC‑conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PLZF
Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.
  • Long Name:
    Promyelocytic Leukemia Zinc Finger
  • Entrez Gene IDs:
    7704 (Human); 235320 (Mouse)
  • Alternate Names:
    PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. MAIT cells are reduced in frequency and functionally impaired in human T lymphotropic virus type 1 infection: Potential clinical implications
    Authors: D Paquin-Pro, BC Greenspun, EA Costa, AC Segurado, EG Kallas, DF Nixon, FE Leal
    PLoS ONE, 2017;12(4):e0175345.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development.
    Authors: Leeansyah E, Loh L, Nixon D, Sandberg J
    Nat Commun, 2014;5(0):3143.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Flow
  3. TLR-induced cytokines promote effective proinflammatory natural Th17 cell responses.
    Authors: Massot B, Michel M, Diem S, Ohnmacht C, Latour S, Dy M, Eberl G, Leite-de-Moraes M
    J Immunol, 2014;192(12):5635-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Human iNKT and MAIT cells exhibit a PLZF-dependent proapoptotic propensity that is counterbalanced by XIAP.
    Authors: Gerart S, Siberil S, Martin E, Lenoir C, Aguilar C, Picard C, Lantz O, Fischer A, Latour S
    Blood, 2013;121(4):614-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
    Authors: Leeansyah E, Ganesh A, Quigley M, Sonnerborg A, Andersson J, Hunt P, Somsouk M, Deeks S, Martin J, Moll M, Shacklett B, Sandberg J
    Blood, 2013;121(7):1124-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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