Human Podoplanin APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Podoplanin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse Podoplanin is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Podoplanin
    Accession # Q86YL7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Podoplanin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Sheep Anti-Human Podoplanin APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3670A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Podoplanin

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family (1‑2). Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207‑228) and a short cytoplasmic tail (aa 229‑238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation (2‑3). There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4‑5), and numerous tumors, including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8‑10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).

  • References:
    1. Zimmer, G. et al. (1999) Biochem. J. 341:277.
    2. Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
    3. Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
    4. Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
    5. Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
    6. Kato, Y. et al. (2005) Tumour Biol. 26:195.
    7. Kato, Y. et al. (2004) Oncogene 23:8552.
    8. Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
    9. Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
    10. Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
  • Long Name:
    Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
  • Entrez Gene IDs:
    10630 (Human); 14726 (Mouse); 54320 (Rat)
  • Alternate Names:
    36-KD; aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG APC-conjugated Antibody

Ctrl IC016A 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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