Human POU3F2 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7427U Datasheet / COA / SDS

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FAB7427U-100UG

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Human POU3F2 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.

Source

Monoclonal Mouse IgG2a Clone # 761340

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human POU3F2
Asn7-His59
Accession # P20265

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: POU3F2

POU3F2, also called Brn2, is a 47 kDa (predicted) member of the Octamer-binding transcription factor family. It is mainly expressed in the brain, especially in neuroectodermal cell lineages. With transcription factors ASCL1/Mash1 and Myt1L, POU3F2 has been used to induce neuronal differentiation of human stem cells and mouse fibroblasts. The full length transcript of POU3F2 produces the transcription factor N-Oct3, while alternate transcription initiation at amino acids 181 and 200 produce N-Oct5A and N-Oct5B isoforms, respectively. The sequence used as an immunogen is present only in N-Oct3 and is identical in human and mouse, while rat POU3F2 shows one amino acid difference.

Long Name

POU Class 3 Homeobox 2

Entrez Gene IDs

5454 (Human)

Alternate Names

Brain-2; Brain-specific homeobox/POU domain protein 2; BRN2; brn-2; BRN2brain-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct7; oct-7; OCT7POU domain class 3, transcription factor 2; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF7; OTF-7; POU class 3 homeobox 2; POU domain, class 3, transcription factor 2; POU3F2; POUF3