Detection of Human POU3F2 by Western Blot.
Western blot shows lysates of A375 human melanoma cell line, Bowes human melanoma cell line, and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POU3F2 Monoclonal Antibody (Catalog # MAB7427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for POU3F2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
POU3F2, also called Brn2, is a 47 kDa (predicted) member of the Octamer-binding transcription factor family. It is mainly expressed in the brain, especially in neuroectodermal cell lineages. With transcription factors ASCL1/Mash1 and Myt1L, POU3F2 has been used to induce neuronal differentiation of human stem cells and mouse fibroblasts. The full length transcript of POU3F2 produces the transcription factor N-Oct3, while alternate transcription initiation at amino acids 181 and 200 produce N-Oct5A and N-Oct5B isoforms, respectively. The sequence used as an immunogen is present only in N-Oct3 and is identical in human and mouse, while rat POU3F2 shows one amino acid difference.
POU Class 3 Homeobox 2
Entrez Gene IDs:
Brain-2; Brain-specific homeobox/POU domain protein 2; BRN2; brn-2; BRN2brain-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct7; oct-7; OCT7POU domain class 3, transcription factor 2; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF7; OTF-7; POU class 3 homeobox 2; POU domain, class 3, transcription factor 2; POU3F2; POUF3
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