Human/Primate Dopamine beta-Hydroxylase N-Terminus Antibody Summary
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Detection of Dopamine beta ‑Hydroxylase (N-Terminus). Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein.
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Background: Dopamine beta-Hydroxylase
Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu+-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl- concentrations.
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Citation for Human/Primate Dopamine beta-Hydroxylase N-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons.
Authors: Chernova T, Steinert JR, Richards P
Mol. Pharmacol., 2011;79(5):844-54.
Sample Types: Cell Lysates
Applications: Western Blot
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