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Human Profilin 1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7779V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7779V-100UG

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Human Profilin 1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Profilin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Profilin 2 is observed.
Source
Monoclonal Mouse IgG2a Clone # 816536
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human Profilin 1
Met1-Tyr140
Accession # P07737
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Profilin 1

Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.

Entrez Gene IDs
5216 (Human); 18643 (Mouse)
Alternate Names
PFN1; PROF1; Profilin 1; Profilin I; profilin-1

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