|Detection of Human and Mouse Profilin 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Profilin 1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.
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