Human Progranulin Quantikine ELISA Kit

  (8 citations)
(1 Review)
    
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Assay Procedure
Citations (8)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL), Human Milk (10 uL)
  • Sensitivity
    0.54 ng/mL
  • Assay Range
    1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC147 , Quantikine Immunoassay Control Set 612 for Human Progranulin - Please Inquire
Product Summary
The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 13.5 32.7 64.8 14.4 33.1 64.4
Standard Deviation 0.7 1.9 4.1 1 2.3 4.4
CV% 5.2 5.8 6.3 6.9 6.9 6.8

Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 15.5 36.6 70.8 15.7 35.8 69.1
Standard Deviation 0.9 1.7 3.1 1.3 2.9 5.1
CV% 5.8 4.6 4.4 8.3 8.1 7.4

Recovery

The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 103-112
EDTA Plasma (n=4) 102 95-110
Heparin Plasma (n=4) 100 92-108
Serum (n=4) 104 90-115
Urine (n=4) 109 99-116
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Progranulin/PGRN [HRP]
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Progranulin/PGRN
Progranulin, also known as proepithelin and PC cell-derived growth factor (PCDGF), is a secreted, glycosylated protein with multiple repeats of the granulin/epithelin motif.
  • Entrez Gene IDs:
    2896 (Human); 14824 (Mouse)
  • Alternate Names:
    Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Sample Type
  1. Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients
    Alzheimers Res Ther, 2016;8(1):43.
    Species: Human
    Sample Type: Plasma
  2. Serum levels of the adipokine progranulin depend on renal function.
    Authors: Richter J, Focke D, Ebert T, Kovacs P, Bachmann A, Lossner U, Kralisch S, Kratzsch J, Beige J, Anders M, Bast I, Bluher M, Stumvoll M, Fasshauer M
    Diabetes Care, 2013;36(2):410-4.
    Species: Human
    Sample Type: Serum
  3. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
    Biochem Biophys Res Commun, 2012;430(1):419-24.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  5. Progranulin regulates neuronal outgrowth independent of sortilin.
    Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
    Mol Neurodegener, 2012;7(0):33.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Low plasma progranulin levels in children with autism.
    Authors: Al-Ayadhi LY, Mostafa GA,
    J Neuroinflammation, 2011;8(0):111.
    Species: Human
    Sample Type: Plasma
  7. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
    Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
    Clin. Cancer Res., 2011;17(8):2395-2399.
    Species: Human
    Sample Type: Serum
  8. Genome-wide screen identifies rs646776 near sortilin as a regulator of progranulin levels in human plasma.
    Authors: Carrasquillo MM, Nicholson AM, Finch N, Gibbs JR, Baker M, Rutherford NJ, Hunter TA, DeJesus-Hernandez M, Bisceglio GD, Mackenzie IR, Singleton A, Cookson MR, Crook JE, Dillman A, Hernandez D, Petersen RC, Graff-Radford NR, Younkin SG, Rademakers R
    Am. J. Hum. Genet., 2010;87(6):890-7.
    Species: Human
    Sample Type: Plasma

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Quantikine Immunoassay Control Set 612 for Human Progranulin

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Quantikine ELISA Wash Buffer 1

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Average Rating: 5 (Based on 1 review)

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 Human Progranulin Quantikine ELISA Kit DPGRN0 Array DPGRN0
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 Human Progranulin Quantikine ELISA Kit DPGRN0
: Human Progranulin Quantikine ELISA Kit [DPGRN0].

Summary

Sample Tested Cell culture supernatant

Other Experimental Details

Other Experimental Details We used device loading with cells that can release progranulin. After certain time in in vivo we explant device and collect culture supernatant for the measurement of progranulin. The kit standard curve was very good as R2 was 1. We can easily detect in our samples.

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