Human Progranulin Quantikine ELISA Kit

Not Available in Europe
Catalog # Availability Size / Price Qty
DPGRN0
Control Products Available
Human Progranulin/PGRN ELISA Serum/Plasma/Urine Human Milk Standard Curve
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Product Details
Procedure
Citations (16)
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Human Progranulin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL), Human Milk (10 uL)
Sensitivity
0.54 ng/mL
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 13.5 32.7 64.8 14.4 33.1 64.4
Standard Deviation 0.7 1.9 4.1 1 2.3 4.4
CV% 5.2 5.8 6.3 6.9 6.9 6.8

Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 15.5 36.6 70.8 15.7 35.8 69.1
Standard Deviation 0.9 1.7 3.1 1.3 2.9 5.1
CV% 5.8 4.6 4.4 8.3 8.1 7.4

Recovery

The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 103-112
EDTA Plasma (n=4) 102 95-110
Heparin Plasma (n=4) 100 92-108
Serum (n=4) 104 90-115
Urine (n=4) 109 99-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Progranulin/PGRN ELISA Linearity

Scientific Data

Human Progranulin/PGRN ELISA Serum/Plasma/Urine Human Milk Standard Curve

Human Progranulin/PGRN ELISA Cell Culture Supernate/Saliva Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Progranulin/PGRN

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer’s disease, and motor neuron disease.

Entrez Gene IDs:
2896 (Human); 14824 (Mouse)
Alternate Names:
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Progranulin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients
    Authors: T Gohda, M Murakoshi, Y Suzuki, M Hiki, T Naito, K Takahashi, Y Tabe
    PLoS ONE, 2022;17(10):e0275745.
    Species: Human
    Sample Types: Serum
  2. Elevated levels of neutrophil gelatinase-associated lipocalin among OCD patients: an exploratory study
    Authors: C Raposo-Lim, IM Pereira, F Marques, P Morgado
    BMC Psychiatry, 2021;21(1):272.
    Species: Human
    Sample Types: Serum
  3. Secretory leukocyte protease inhibitor and progranulin as possible regulators of cervical remodeling in pregnancy
    Authors: T Samejima, T Nagamatsu, N Akiba, T Fujii, S Sayama, K Kawana, A Taguchi, K Kumasawa, T Iriyama, Y Osuga, T Fujii
    J Reprod Immunol, 2020;143(0):103241.
    Species: Human
    Sample Types: Cervical Mucus
  4. Progranulin as a novel biomarker in diagnosis of early-onset neonatal sepsis
    Authors: L Rao, Z Song, X Yu, Q Tu, Y He, Y Luo, Y Yin, D Chen
    Cytokine, 2020;128(0):155000.
    Species: Human
    Sample Types: Serum
  5. Serum progranulin levels are associated with frailty in middle-aged individuals
    Authors: AD Nguyen, TK Malmstrom, ML Niehoff, A Aziz, DK Miller, JE Morley
    PLoS ONE, 2020;15(9):e0238877.
    Species: Human
    Sample Types: Serum
  6. Progranulin deficiency confers resistance to autoimmune encephalomyelitis in mice
    Authors: K Schmitz, A Wilken-Sch, V Vasic, R Brunkhorst, M Schmidt, I Tegeder
    Cell. Mol. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Serum
  7. Role of clusterin/progranulin in toluene diisocyanate-induced occupational asthma
    Authors: GS Choi, HKT Trinh, EM Yang, YM Ye, YS Shin, SH Kim, HS Park
    Exp. Mol. Med., 2018;50(5):58.
    Species: Human
    Sample Types: Serum
  8. Association between progranulin serum levels and dietary intake
    Authors: BB Nicoletto, RA Sarmento, EF Pedrollo, TC Krolikowsk, LH Canani
    PLoS ONE, 2018;13(8):e0202149.
    Species: Human
    Sample Types: Serum
  9. Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients
    Alzheimers Res Ther, 2016;8(1):43.
    Species: Human
    Sample Types: Plasma
  10. Serum levels of the adipokine progranulin depend on renal function.
    Authors: Richter J, Focke D, Ebert T, Kovacs P, Bachmann A, Lossner U, Kralisch S, Kratzsch J, Beige J, Anders M, Bast I, Bluher M, Stumvoll M, Fasshauer M
    Diabetes Care, 2013;36(2):410-4.
    Species: Human
    Sample Types: Serum
  11. p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
    Biochem Biophys Res Commun, 2012;430(1):419-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Progranulin regulates neuronal outgrowth independent of sortilin.
    Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
    Mol Neurodegener, 2012;7(0):33.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Low plasma progranulin levels in children with autism.
    Authors: Al-Ayadhi LY, Mostafa GA,
    J Neuroinflammation, 2011;8(0):111.
    Species: Human
    Sample Types: Plasma
  15. Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
    Authors: Diamandis EP, Goodglick L, Planque C, Thornquist MD
    Clin. Cancer Res., 2011;17(8):2395-2399.
    Species: Human
    Sample Types: Serum
  16. Genome-wide screen identifies rs646776 near sortilin as a regulator of progranulin levels in human plasma.
    Authors: Carrasquillo MM, Nicholson AM, Finch N, Gibbs JR, Baker M, Rutherford NJ, Hunter TA, DeJesus-Hernandez M, Bisceglio GD, Mackenzie IR, Singleton A, Cookson MR, Crook JE, Dillman A, Hernandez D, Petersen RC, Graff-Radford NR, Younkin SG, Rademakers R
    Am. J. Hum. Genet., 2010;87(6):890-7.
    Species: Human
    Sample Types: Plasma

FAQs

  1. What isoforms does this kit detect?

    • This kit detects isoform 1. R&D Systems has not been able to evaluate isoforms 2 and 3 in this kit.

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ELISA Controls

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Reviews for Human Progranulin Quantikine ELISA Kit

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Human Progranulin Quantikine ELISA Kit
By Anonymous on 08/29/2017
Sample Tested: Cell culture supernatant

We used device loading with cells that can release progranulin. After certain time in in vivo we explant device and collect culture supernatant for the measurement of progranulin. The kit standard curve was very good as R2 was 1. We can easily detect in our samples.