Human Protocadherin-19 Antibody

Catalog # Availability Size / Price Qty
MAB8626
MAB8626-SP
Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry.
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Human Protocadherin-19 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Protocadherin-19 in direct ELISA and flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 921614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Protocadherin-19
Met1-Ser678
Accession # Q8TAB3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. View Larger

Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Protocadherin-19 Monoclonal Antibody (Catalog # MAB8626, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-19

Protocadherin 19 (PCDH19) is a d2-group member of the of the protocadherin subfamily, cadherin family of molecules.  PCDH19 is thought to be a calcium-dependent protein primarily expressed in the brain.  The protein is also known as EFMR (Epilepsy Female-restricted with Mental Retardation).

Entrez Gene IDs
57526 (Human); 279653 (Mouse)
Alternate Names
EFMR; EIEE9; PCDH19; Protocadherin19; Protocadherin-19

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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