Human Protocadherin-19 Antibody

R&D Systems | Catalog # MAB8626

R&D Systems
Discontinued Product
MAB8626 has been discontinued. View all Protocadherin-19 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 921614
View all Protocadherin-19 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Protocadherin-19
Met1-Ser678
Accession # Q8TAB3

Specificity

Detects human Protocadherin-19 in direct ELISA and flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Protocadherin-19 Antibody

Detection of Protocadherin-19 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry.

Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Protocadherin-19 Monoclonal Antibody (Catalog # MAB8626, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of Protocadherin-19 in Jurkat cells by Flow Cytometry.

Jurkat cells were stained with Mouse Anti-Human Protocadherin-19 Monoclonal Antibody (Catalog # MAB8626, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Protocadherin-19 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: SH‑SY5Y human neuroblastoma cells and Jurkat cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin-19

Protocadherin 19 (PCDH19) is a d2-group member of the of the protocadherin subfamily, cadherin family of molecules.  PCDH19 is thought to be a calcium-dependent protein primarily expressed in the brain.  The protein is also known as EFMR (Epilepsy Female-restricted with Mental Retardation).

Alternate Names

EFMR, EIEE9, PCDH19, Protocadherin19

Entrez Gene IDs

57526 (Human); 279653 (Mouse)

Gene Symbol

PCDH19

UniProt

Q8TAB3

Additional Protocadherin-19 Products

Product Documents for Human Protocadherin-19 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protocadherin-19 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

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