Human PSCA Antibody Summary
Accession # O43653
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
PSCA in Human Prostate. PSCA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human PSCA Monoclonal Antibody (Catalog # MAB7636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein of the Thy-1/Ly-6 family. PSCA is expressed in differentiating cells and epithelial cells of the prostate, bladder, kidney, skin, esophagus, stomach and placenta. It may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. Mature human PSCA (amino acids 21-95) shares approximately 65% amino acid sequence identity with mouse and rat PSCA.
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