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Human PSCA Antibody

Catalog #: MAB7636
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB7636 has been discontinued.
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PSCA in Human Prostate.
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Citations (1)
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Human PSCA Antibody Summary

Species Reactivity
Human
Specificity
Detects human PSCA in direct ELISAs.
Source
Monoclonal Mouse IgM Clone # 741009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PSCA
Leu21-Ser95
Accession # O43653
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PSCA antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

PSCA in Human Prostate. PSCA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human PSCA Monoclonal Antibody (Catalog # MAB7636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSCA

PSCA (prostate stem cell antigen) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein of the Thy-1/Ly-6 family. PSCA is expressed in differentiating cells and epithelial cells of the prostate, bladder, kidney, skin, esophagus, stomach and placenta. It may be up- or down-regulated in cancer and may promote or suppress the tumor, depending on location. Mature human PSCA (amino acids 21-95) shares approximately 65% amino acid sequence identity with mouse and rat PSCA.

Long Name
Prostate Stem Cell Antigen
Entrez Gene IDs
8000 (Human); 72373 (Mouse)
Alternate Names
PRO232; prostate stem cell antigen; PSCA

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Citation for Human PSCA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells
    Authors: Tomoaki Furuta, Tatsuya Oda, Kayo Kiyoi, Ozawa Yusuke, Sota Kimura, Ko Kurimori et al.
    Cancer Science
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry

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