Human PSGL-1/CD162 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human PSGL-1/CD162 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 688101
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human PSGL-1/CD162
    Accession # NP_002997
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PSGL‑1/CD162 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human PSGL‑1/CD162 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9961G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PSGL-1/CD162

Human PSGL-1 (P-Selectin Glycoprotein Ligand-1; also known as CD162), is a 120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). It is synthesized as a 412 amino acid (aa) preproprecursor that contains a 17 aa signal sequence, a 24 aa pro-peptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (4, 5). Following cleavage of the pre- and pro-segments, it is expressed as a 240 kDa disulfide-linked homodimer. The extreme N-terminus (aa 1-16 of the mature molecule) contains one threonine (aa 16) and three tyrosines (aa 5, 7, and 10) that are involved in ligand binding. The Thr residue allows for O-linked glycosylation in the form of a core-2 structure (GalNAc-Gal) linked in a beta 1,6 bond to a sialylated Lewis X motif (GlcNAc linked to both Fuc and Gal with a terminal sialic acid residue) (1, 2, 5, 6, 7). The three tyrosine residues allow for sulfation (8, 9). When binding to P-Selectin, Tyr sulfation and glycosylation are essential. Tyr7 provides the most efficient sulfate moiety, while Fuc and sialic acid are essentially mandatory (7). When binding to E-Selectin, only carbohydrate is needed, while both carbohydrate and Tyr10 are used for L-Selectin binding (6, 8). There are 16 decameric aa repeats in the ECD of the longform of PSGL-1. This form is referred to as the A allele, and represents 65 - 80% of the population. Alleles B and C show deletions of decameric repeats #2 (aa 132-141) plus #9 and 10 (aa 222-241), respectively. Shorter forms may show weaker binding to P-Selectin (9, 10). Soluble forms of PSGL-1 are also known. Neutrophil Elastase will cleave somewhere within repeats #5-9, while Cathepsin G cleaves after Tyr7 (11). The loss of Tyr5 and 7 should impact binding affinity. PSGL-1 is found on virtually all leukocytes and macrophages/DCs (1). Although there is similarity in the organization of the ECD between species, there is little aa identity. Human PSGL-1 ECD shares 51%, 52% and 43% aa sequence identity with equine, canine and mouse ECD, respectively.

  • References:
    1. Yang, J. et al. (1999) Thromb. Haemost. 81:1.
    2. Cummings, R.D. (1999) Braz. J. Med. Biol. Res. 32:519.
    3. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
    4. Sako, D. et al. (1993) Cell 75:1179.
    5. Veldman, G.M. et al. (1995) J. Biol. Chem. 270:16470.
    6. Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
    7. Leppanen, A. et al. (2000) J. Biol. Chem. 275:39569.
    8. Sako, D. et al. (1995) Cell 83:323.
    9. Afshar-Kharghan, V. et al. (2001) Blood 97:3306.
    10. Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
    11. Gardiner, E.E. et al. (2001) Blood 98:1440.
  • Long Name:
    P-Selectin Glycoprotein Ligand 1
  • Entrez Gene IDs:
    6404 (Human); 20345 (Mouse); 363930 (Rat)
  • Alternate Names:
    CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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