Human RAMP3 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF4875AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Specificity

Detects human RAMP3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RAMP1, rhRAMP2, and recombinant mouse RAMP2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human RAMP3 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RAMP3

RAMP3 (receptor activity modifying protein 3) is a 20-26 kDa member of the RAMP family of proteins. It is expressed on multiple cell types in conjunction with CRLR and NHERF-1, thereby generating a receptor complex for adrenomedullin (AM). Mature human RAMP3 is a 125 amino acid (aa) type I transmembrane glycoprotein that contains a 95 aa extracellular domain (ECD) (aa 24-118) and a 10 aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 59-65 are critical for AM binding. There is one potential splice variant that shows a 49 aa substitution for the first 19 aa of the signal sequence. The human ECD is 88% aa identical to the mouse ECD. Members of RAMP family of proteins are known to form complexes with apparent molecular weights of 25 to 50 kDa that are resistant to denaturing and reducing agents.

Long Name

Receptor Activity Modifying Protein 3

Alternate Names

calcitonin receptor-like receptor activity modifying protein 3, Calcitonin-receptor-like receptor activity-modifying protein 3, CRLR activity-modifying protein 3, receptor (calcitonin) activity modifying protein 3, receptor (G protein-coupled) activity modifying protein 3, receptor activity modifying protein 3, receptor activity-modifying protein 3, receptor-activity-modifying protein 3

Entrez Gene IDs

10268 (Human); 56089 (Mouse); 56820 (Rat)

Gene Symbol

RAMP3

UniProt

Additional RAMP3 Products

Product Documents for Human RAMP3 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RAMP3 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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