Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all RAMP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RAMP3
Arg24-Val118
Accession # O60896

Specificity

Detects human RAMP3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RAMP1, rhRAMP2, and recombinant mouse RAMP2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RAMP3 Antibody

Detection of Human RAMP3 antibody by Western Blot.

Detection of Human RAMP3 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4875) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human RAMP3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and human brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RAMP3

RAMP3 (receptor activity modifying protein 3) is a 20-26 kDa member of the RAMP family of proteins. It is expressed on multiple cell types in conjunction with CRLR and NHERF-1, thereby generating a receptor complex for adrenomedullin (AM). Mature human RAMP3 is a 125 amino acid (aa) type I transmembrane glycoprotein that contains a 95 aa extracellular domain (ECD) (aa 24-118) and a 10 aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 59-65 are critical for AM binding. There is one potential splice variant that shows a 49 aa substitution for the first 19 aa of the signal sequence. The human ECD is 88% aa identical to the mouse ECD. Members of RAMP family of proteins are known to form complexes with apparent molecular weights of 25 to 50 kDa that are resistant to denaturing and reducing agents.

Long Name

Receptor Activity Modifying Protein 3

Alternate Names

calcitonin receptor-like receptor activity modifying protein 3, Calcitonin-receptor-like receptor activity-modifying protein 3, CRLR activity-modifying protein 3, receptor (calcitonin) activity modifying protein 3, receptor (G protein-coupled) activity modifying protein 3, receptor activity modifying protein 3, receptor activity-modifying protein 3, receptor-activity-modifying protein 3

Entrez Gene IDs

10268 (Human); 56089 (Mouse); 56820 (Rat)

Gene Symbol

RAMP3

UniProt

O60896

Additional RAMP3 Products

Product Documents for Human RAMP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RAMP3 Antibody

For research use only

Citations for Human RAMP3 Antibody

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