RARRES1 (retinoic acid receptor responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Human RARRES1 is 294 amino acids (aa) in length. It is a type II transmembrane (TM) protein with a TM segment between aa 21-42. Three potential splice variants exist. There is a 46 aa N terminal extension, a four aa substitution for aa 225-294 and a combination of these two variants. Over aa 43-294, human RARRES1 shares 75% aa identity with mouse RARRES1.
Discontinued Product
AF4255 has been discontinued.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human RARRES1
Pro43-Phe294
Accession # P49788
Pro43-Phe294
Accession # P49788
Specificity
Detects human RARRES1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RARRES1 Antibody
Detection of Human RARRES1 by Western Blot.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RARRES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4255) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RARRES1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human RARRES1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Reviewed Applications
Read 1 review rated 5 using AF4255 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RARRES1
Long Name
Retinoic Acid Receptor Responder [Tazarotene-induced] 1
Alternate Names
TIG1
Gene Symbol
RARRES1
UniProt
Additional RARRES1 Products
Product Documents for Human RARRES1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human RARRES1 Antibody
For research use only
Related Research Areas
Citations for Human RARRES1 Antibody
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Application: ImmunocytochemistrySample Tested: SUM-149 xenografts miceSpecies: HumanVerified Customer | Posted 05/01/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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