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Human RARRES1 Antibody

Catalog #: AF4255
5 Citations Datasheet / COA / SDS

Discontinued Product

AF4255 has been discontinued.
View all RARRES1 products.
Detection of Human RARRES1 by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RARRES1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RARRES1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RARRES1
Pro43-Phe294
Accession # P49788
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RARRES1 antibody by Western Blot. View Larger

Detection of Human RARRES1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RARRES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4255) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RARRES1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RARRES1

RARRES1 (retinoic acid receptor responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Human RARRES1 is 294 amino acids (aa) in length. It is a type II transmembrane (TM) protein with a TM segment between aa 21-42. Three potential splice variants exist. There is a 46 aa N terminal extension, a four aa substitution for aa 225-294 and a combination of these two variants. Over aa 43-294, human RARRES1 shares 75% aa identity with mouse RARRES1.

Long Name
Retinoic Acid Receptor Responder [Tazarotene-induced] 1
Entrez Gene IDs
5918 (Human); 109222 (Mouse); 310486 (Rat)
Alternate Names
RARRES1; RAR-responsive protein TIG1; retinoic acid receptor responder (tazarotene induced) 1; retinoic acid receptor responder protein 1; TIG1; TIG1Tazarotene-induced gene 1 protein

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Citations for Human RARRES1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression
    Authors: Anqun Chen, Ye Feng, Han Lai, Wenjun Ju, Zhengzhe Li, Yu Li et al.
    Journal of Clinical Investigation
  2. Secretome Survey of Human Plexiform Neurofibroma Derived Schwann Cells Reveals a Secreted form of the RARRES1 Protein
    Authors: Hui-Ling Chen, Haeri Seol, Kristy Jean Brown, Heather Gordish-Dressman, Ashley Hill, Vittorio Gallo et al.
    International Journal of Molecular Sciences
  3. Hypermethylation and loss of retinoic acid receptor responder 1 expression in human choriocarcinoma
    Authors: H Huebner, R Strick, DL Wachter, S Kehl, PL Strissel, R Schneider-, A Hartner, W Rascher, LC Horn, MW Beckmann, M Ruebner, FB Fahlbusch
    J. Exp. Clin. Cancer Res., 2017-11-23;36(1):165.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Epigenetic repression of RARRES1 is mediated by methylation of a proximal promoter and a loss of CTCF binding.
    Authors: Peng Z, Shen R, Li YW
    PLoS ONE, 2012-05-17;7(5):e36891.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Tumor Suppressor RARRES1 Interacts with Cytoplasmic Carboxypeptidase AGBL2 to Regulate the alpha-Tubulin Tyrosination Cycle.
    Authors: Sahab ZJ, Hall MD, Me Sung Y, Dakshanamurthy S, Ji Y, Kumar D, Byers SW
    Cancer Res., 2011-02-08;71(4):1219-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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