Human/Rat/Primate Phospho-GRIN2B/NMDAR2B (Y1252) Antibody

Catalog # Availability Size / Price Qty
PPS056
Detection of GRIN2B/NMDAR2B (Y1252) by Western Blot.
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Human/Rat/Primate Phospho-GRIN2B/NMDAR2B (Y1252) Antibody Summary

Species Reactivity
Human, Rat, Primate
Specificity
Human, non-human primates, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1252 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1252 of NMDA R, NR2B Subunit
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of GRIN2B/NMDAR2B (Y1252) antibody by Western Blot. View Larger

Detection of GRIN2B/NMDAR2B (Y1252) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1252 (Control). The phosphospecificity of this labeling is shown in the second lane ( lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1252). The immunolabeling is completely eliminated by treatment with lambda PPase.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluR epsilon 2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.

References
  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
  5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
  6. Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
Entrez Gene IDs
2904 (Human)
Alternate Names
GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B

Product Datasheets

Citation for Human/Rat/Primate Phospho-GRIN2B/NMDAR2B (Y1252) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons.
    Authors: Chernova T, Steinert JR, Richards P
    Mol. Pharmacol., 2011;79(5):844-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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