Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Rad17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rad17
Asn2-Arg240
Accession # O75943

Specificity

Detects human and rat Rad17 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Rat Rad17 Antibody

Detection of Human and Rat Rad17 antibody by Western Blot.

Detection of Human and Rat Rad17 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rad17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Rat Rad17 Antibody

Application
Recommended Usage

Immunoprecipitation

3 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rad17

The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).

Alternate Names

CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog

Entrez Gene IDs

5884 (Human); 310034 (Rat)

Gene Symbol

RAD17

UniProt

O75943

Additional Rad17 Products

Product Documents for Human/Rat Rad17 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Rat Rad17 Antibody

For research use only

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