Human Reduced Folate Carrier/SLC19A1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8450A-025
Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human SLC19A1 and eGFP by Flow Cytometry.
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Human Reduced Folate Carrier/SLC19A1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human Reduced Folate Carrier/SLC19A1 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 890513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Reduced Folate Carrier/SLC19A1
Met1-Gln591
Accession # P41440
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human SLC19A1 and eGFP by Flow Cytometry. View Larger

Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human SLC19A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC19A1 and eGFP were stained with either (A) Mouse Anti-Human Reduced Folate Carrier/SLC19A1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8450A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Reduced Folate Carrier/SLC19A1

SLC19A1, also known as Reduced Folate Carrier protein, is a multi-pass transmembrane transporter that is involved in the regulation of intracellular concentrations of folate. There is an association between idiopathic Recurrent Spontaneous Abortion (RSA) and SLC19A1 polymorphisms that may be useful biomarkers for RSA risk. Three transcript variants encoding different isoforms have been found for this gene. Human and mouse SLC19A1 share only 58% amino acid (aa) sequence identity, with considerable divergence over the C-terminal 100 aa.

Long Name
Solute Carrier Family 12 (Folate Transporter) Member 3
Entrez Gene IDs
6573 (Human); 20509 (Mouse); 29723 (Rat)
Alternate Names
CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1

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