Human Reduced Folate Carrier/SLC19A1 APC-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Stains human Reduced Folate Carrier/SLC19A1 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 890513
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Reduced Folate Carrier/SLC19A1
    Met1-Gln591
    Accession # P41440
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human SLC19A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC19A1 and eGFP were stained with either (A) Mouse Anti-Human Reduced Folate Carrier/SLC19A1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8450A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Reduced Folate Carrier/SLC19A1
SLC19A1, also known as Reduced Folate Carrier protein, is a multi-pass transmembrane transporter that is involved in the regulation of intracellular concentrations of folate. There is an association between idiopathic Recurrent Spontaneous Abortion (RSA) and SLC19A1 polymorphisms that may be useful biomarkers for RSA risk. Three transcript variants encoding different isoforms have been found for this gene. Human and mouse SLC19A1 share only 58% amino acid (aa) sequence identity, with considerable divergence over the C-terminal 100 aa.
  • Long Name:
    Solute Carrier Family 12 (Folate Transporter) Member 3
  • Entrez Gene IDs:
    6573 (Human); 20509 (Mouse); 29723 (Rat)
  • Alternate Names:
    CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 9
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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