Human Reduced Folate Carrier/SLC19A1 Antibody

Catalog # Availability Size / Price Qty
MAB8450
MAB8450-SP

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Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP by Flow Cytometry.
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Human Reduced Folate Carrier/SLC19A1 Antibody Summary

Species Reactivity
Human
Specificity
Stains human Reduced Folate Carrier/SLC19A1 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 890513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Reduced Folate Carrier/SLC19A1
Met1-Gln591
Accession # P41440
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Reduced Folate Carrier/SLC19A1 antibody in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP antibody by Flow Cytometry. View Larger

Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human Reduced Folate Carrier/SLC19A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Reduced Folate Carrier/SLC19A1 Monoclonal Antibody (Catalog # MAB8450) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reduced Folate Carrier/SLC19A1

SLC19A1, also known as, Reduced Folate Carriers, is a transmembrane transporter that is involved in the regulation of intracellular concentrations of folate. There is an association between idiopathic recurrent spontaneous abortion (RSA) and 3 SLC19A1 polymorphisms that may be useful biomarkers for RSA risk. Three transcript variants encoding different isoforms have been found for this gene.

Long Name
Solute Carrier Family 12 (Folate Transporter) Member 3
Entrez Gene IDs
6573 (Human); 20509 (Mouse); 29723 (Rat)
Alternate Names
CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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