Human Reg1A Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Reg1A in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Reg1B, rhReg4, recombinant rat (rr) Reg2, or rrReg3.
  • Source
    Monoclonal Rat IgG2B Clone # 431202
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Reg1A
    Accession # P05451
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Reg1A in AGS Human Cell Line by Flow Cytometry. AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4937G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Reg1A

Human Reg1A, also known as Reg1 alpha, PTP, PSP and Lithostathine, is a 16-22 kDa, variably glycosylated member of the Reg (Regenerating) family, C-type lectin superfamily of molecules. It is further classified as a type I subfamily member based on gene organization and expression pattern. These are four Reg subfamilies spread across multiple species, with three (or four) additional human Reg family genes assigned to three of the subfamilies. Reg1A is secreted, and circulates as either a monomer, dimer, or tetramer. Once secreted, Reg1A may undergo proteolysis with removal of the N-terminal 11 amino acids (aa). This renders it insoluble with subsequent fibril formation. Reg1A is typically inducible, and human cells reported to express Reg1A include pancreatic islet beta -cells, neurons, intestinal Paneth cells, stomach Chief cells, cardiomyocytes, salivary duct epithelium and antral endocrine cells of the stomach. Reg1A has proliferative effects on multiple cell types, and likely serves as an anti-inflammatory agent. This is presumably mediated by binding to its receptor, EXTL3. Mature human Reg1A (aa 23-166) shares 75%, 69% and 88% aa sequence identity with mouse RegI,rat Reg, and human Reg1B, respectively.

  • Long Name:
    Regenerating Islet-derived 1A
  • Entrez Gene IDs:
    5967 (Human)
  • Alternate Names:
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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