Human Reg1A, also known as Reg1 alpha, PTP, PSP and Lithostathine, is a 16-22 kDa, variably glycosylated member of the Reg (Regenerating) family, C-type lectin superfamily of molecules. It is further classified as a type I subfamily member based on gene organization and expression pattern. These are four Reg subfamilies spread across multiple species, with three (or four) additional human Reg family genes assigned to three of the subfamilies. Reg1A is secreted, and circulates as either a monomer, dimer, or tetramer. Once secreted, Reg1A may undergo proteolysis with removal of the N-terminal 11 amino acids (aa). This renders it insoluble with subsequent fibril formation. Reg1A is typically inducible, and human cells reported to express Reg1A include pancreatic islet beta -cells, neurons, intestinal Paneth cells, stomach Chief cells, cardiomyocytes, salivary duct epithelium and antral endocrine cells of the stomach. Reg1A has proliferative effects on multiple cell types, and likely serves as an anti-inflammatory agent. This is presumably mediated by binding to its receptor, EXTL3. Mature human Reg1A (aa 23-166) shares 75%, 69% and 88% aa sequence identity with mouse RegI,rat Reg, and human Reg1B, respectively.
Human Reg1A Alexa Fluor® 488‑conjugated Antibody
R&D Systems | Catalog # IC4937G
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gln23-Asn166
Accession # P05451
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Reg1A Alexa Fluor® 488‑conjugated Antibody
Detection of Reg1A in AGS Human Cell Line by Flow Cytometry.
AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4937G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Reg1A Alexa Fluor® 488‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: AGS human gastric adenocarcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
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Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Reg1A
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Reg1A Products
Product Documents for Human Reg1A Alexa Fluor® 488‑conjugated Antibody
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Product Specific Notices for Human Reg1A Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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