Human RELT/TNFRSF19L Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of RELT/TNFRSF19L in HEK293 Human cell line Transfected with Human RELT/TNFRSF19L and eGFP by Flow Cytometry. HEK293 Human cell line transfected with Human RELT/TNFRSF19L and eGFP were stained with (A) Mouse Anti-Human RELT/TNFRSF19L Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB1385T) or (B) Mouse IgG2B Alexa Fluor® 594‑conjugated isotype control antibody ollowed (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T‑cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.

Product Datasheets

Related Research Areas

• Co-stimulatory and Co-inhibitory Molecules
• Regulation of Apoptosis by TNF Superfamily Members
• Regulation of T Cell Co-stimulation by TNF Superfamily Members
• TNF Superfamily
• TNF Superfamily Receptors