Detection of RELT/TNFRSF19L in HEK293 Human cell line Transfected with Human RELT/TNFRSF19L and eGFP by Flow Cytometry.
HEK293 Human cell line transfected with Human RELT/TNFRSF19L and eGFP were stained with (A) Mouse Anti-Human RELT/TNFRSF19L Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB1385T) or (B) Mouse IgG2B Alexa Fluor® 594‑conjugated isotype control antibody ollowed (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 degreesC as supplied.
RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co-stimulate T-cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.
Have you used Human RELT/TNFRSF19L Alexa Fluor® 594-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.