Human RELT/TNFRSF19L Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1385R-100UG

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Detection of RELT/TNFRSF19L in HEK293 Human cell line Transfected with Human RELT/TNFRSF19L and eGFP by Flow Cytometry.
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Human RELT/TNFRSF19L Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human RELT/TNFRSF19L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, recombinant mouse (rm) 4-1BB, rhBAFF R, rmBAFF R, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rmDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rrFas, rhGITR, rmGITR, rhHVEM, rhLT beta  R, rmLT beta  R, rhNGF R, rmNGF R, rhOPG, rmOPG, rmOX40, rhRANK, rmRANK, rhTNF RI, rmTNF RI, rhTNF RII, rmTNF RII, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL R4, rhTROY, or rmTROY is observed.
Source
Monoclonal Mouse IgG2B Clone # 238104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RELT/TNFRSF19L
Met1-Ala160 (Arg127Gly, Arg129Gly)
Accession # Q969Z4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of RELT/TNFRSF19L in HEK293 Human cell line Transfected with Human RELT/TNFRSF19L and eGFP by Flow Cytometry. View Larger

Detection of RELT/TNFRSF19L in HEK293 Human cell line Transfected with Human RELT/TNFRSF19L and eGFP by Flow Cytometry. HEK293 Human cell line transfected with Human RELT/TNFRSF19L and eGFP were stained with (A) Mouse Anti-Human RELT/TNFRSF19L Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1385R) or (B) Mouse IgG2B Alexa Fluor® 647-conjugated isotype control antibody ollowed (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T‑cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.

References
Long Name
Receptor Expressed in Lymphoid Tissues
Entrez Gene IDs
84957 (Human); 320100 (Mouse); 101865743 (Cynomolgus Monkey)
Alternate Names
Receptor expressed in lymphoid tissues; RELT tumor necrosis factor receptor; RELT; TNFRSF19L; TNFRSF19LFLJ14993; tumor necrosis factor receptor superfamily member 19L; tumor necrosis factor receptor superfamily, member 19-like

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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