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Human Renin Quantikine ELISA Kit

R&D Systems | Catalog # DREN00

This assay detects pro- and mature Renin.
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (50 µL)

Sensitivity

14.8 pg/mL

Assay Range

31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human Renin Quantikine ELISA Kit

The Quantikine Human Renin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human mature Renin and Prorenin in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Renin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Renin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Renin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Renin. This assay recognizes both the Pro- and mature versions of recombinant human Renin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human Renin in this assay. No medical histories were available for the donors used in this study.

Sample typeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=35)776232-1892374
EDTA plasma (n=35)726201-1852365
Heparin plasma680179-1760349

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Urine (n=12)60.442ND-96.7
ND=Non-detectable

Cell Culture Supernates - CCD-1070Sk human foreskin fibroblasts were cultured in MEM supplemented with 10% fetal bovine serum and 1 mM sodium pyruvate and grown for 8 days until confluent. An aliquot of the cell culture supernate was removed, assayed for human Renin, and measured 197 pg/mL.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 201 599 1193 191 566 1153
Standard Deviation 10.6 14.2 19.9 10.5 27.1 46.4
CV% 5.3 2.4 1.7 5.5 4.8 4.0

Recovery for Human Renin Quantikine ELISA Kit

The recovery of Renin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 100 93-106
EDTA Plasma (n=4) 101 95-107
Heparin Plasma (n=4) 105 99-114
Serum (n=4) 101 90-109
Urine (n=4) 93 80-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Renin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Renin ELISA Linearity

Scientific Data Images for Human Renin Quantikine ELISA Kit

Human Renin ELISA Standard Curve

Human Renin ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Renin

Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23), a propeptide (aa 24 - 66), and a mature chain (aa 67 - 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.

Mouse Renin is a secreted, 42 - 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.

Alternate Names

Angiotensinogenase, REN

Entrez Gene IDs

5972 (Human); 19701 (Mouse)

Gene Symbol

REN

Additional Renin Products

Product Documents for Human Renin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Renin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Renin Quantikine ELISA Kit

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  • Human Renin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/03/2023
    Measured plasma at 8-fold dilution and urine at 2-fold dilution.
    Human Renin Quantikine ELISA Kit DREN00
  • Human Renin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 08/22/2019
    Human Renin Quantikine ELISA Kit DREN00

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Protocols

View specific protocols for Human Renin Quantikine ELISA Kit (DREN00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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