Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 132507
View all Ret Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ret/Fc Chimera
Leu29-Arg635
Accession # P07949

Specificity

Detects human Ret in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Ret is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Ret APC‑conjugated Antibody

Detection of Ret antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry.

Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Ret APC-conjugated Monoclonal Antibody (Catalog # IC718A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Ret APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: SH‑SY5Y human neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1‑4) (1). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is a 170-175 kDa type I transmembrane glycoprotein that is encoded by the c-ret proto-oncogene (2,3). Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma (4). Human and mouse Ret share 83% amino acid (aa) sequence identity (77% identity in the extracellular domain over aa 29-635). Although Ret does not bind GDNF ligands directly, in general, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (5-6).

References

  1. Wang, X. (2013) Biochim. Biophys. Acta. 1834:2205.
  2. Takahashi, M. and G.M. Cooper (1987) Mol. Cell Biol. 7:1378.
  3. Richardson, D.S. et al. (2012) Mol. Cell Biol. 23:3838.
  4. Mulligan, L.M. et al. (2014) Nat. Rev. Cancer 14:173.
  5. Ibanez, C.F. (2013) Cold Spring Harb. Perspect. Biol. 5:a009134.
  6. Plaza-Menacho, I. et al. (2014) Cell. Signal. 26:1743.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

P07949

Additional Ret Products

Product Documents for Human Ret APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Ret APC‑conjugated Antibody

For research use only

Related Research Areas

Cancer Biomarkers
GDNF Family

Citations for Human Ret APC‑conjugated Antibody

Customer Reviews for Human Ret APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Ret APC‑conjugated Antibody and earn rewards!

Have you used Human Ret APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies