Human Ret APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC718A
Human Ret APC-conjugated Antibody in Data
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Ret APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ret in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Ret is observed.
Source
Monoclonal Mouse IgG1 Clone # 132507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ret/Fc Chimera
Leu29-Arg635
Accession # P07949
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry. View Larger

Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Ret APC-conjugated Monoclonal Antibody (Catalog # IC718A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1‑4) (1). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is a 170-175 kDa type I transmembrane glycoprotein that is encoded by the c-ret proto-oncogene (2,3). Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma (4). Human and mouse Ret share 83% amino acid (aa) sequence identity (77% identity in the extracellular domain over aa 29-635). Although Ret does not bind GDNF ligands directly, in general, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (5-6).

References
  1. Wang, X. (2013) Biochim. Biophys. Acta. 1834:2205.
  2. Takahashi, M. and G.M. Cooper (1987) Mol. Cell Biol. 7:1378.
  3. Richardson, D.S. et al. (2012) Mol. Cell Biol. 23:3838.
  4. Mulligan, L.M. et al. (2014) Nat. Rev. Cancer 14:173.
  5. Ibanez, C.F. (2013) Cold Spring Harb. Perspect. Biol. 5:a009134.
  6. Plaza-Menacho, I. et al. (2014) Cell. Signal. 26:1743.
Long Name
Proto-oncogene Tyrosine-protein Kinase Receptor Ret
Entrez Gene IDs
5979 (Human); 19713 (Mouse)
Alternate Names
CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret

Product Datasheets

Citation for Human Ret APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Ret APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Ret APC-conjugated Antibody and earn rewards!

Have you used Human Ret APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review