Detection of Human RFC1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFC1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
RFC1 (Replication factor C subunit 1; also RFC140) is a 140‑150 kDa, ubiquitously expressed member of the activator 1 large subunit family of proteins. It is one of five RFC complex subunits that participate in the early stages of DNA replication. Following the initiation of DNA synthesis by pol alpha /primase, an RFC complex displaces pol alpha at the replication fork, and recruits a PCNA homotrimeric complex to this site. This interacts with polδ to extend the newly‑formed DNA strand. Human RFC1 is 1148 amino acids (aa) in length. It contains a phosphorylated-DNA binding region (aa 366‑477), a PCNA binding domain (aa 478‑712), an ATPase site (aa 643‑772), and an NLS (aa 1120‑1124). Caspase-3 cleaves RFC-1 into 97, 73 and 65 kDa fragments during apoptosis. There is one potential alternative start site at Met117. Over aa 800‑1093, human RFC1 shares 90% aa identity with mouse RFC1.
Replication Factor C 1
Entrez Gene IDs:
5981 (Human); 19687 (Mouse); 89809 (Rat)
A1 140 kDa subunit; A1; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; MGC51786; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1 (145kD); replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit; RFC; RFC140; RFC140DNA-binding protein PO-GA