Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all RFC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RFC1
Met800-Ser1093
Accession # P35251

Specificity

Detects human RFC1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RFC1 Antibody

Detection of Human RFC1 antibody by Western Blot.

Detection of Human RFC1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFC1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RFC1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RFC1

RFC1 (Replication factor C subunit 1; also RFC140) is a 140‑150 kDa, ubiquitously expressed member of the activator 1 large subunit family of proteins. It is one of five RFC complex subunits that participate in the early stages of DNA replication. Following the initiation of DNA synthesis by pol alpha /primase, an RFC complex displaces pol alpha at the replication fork, and recruits a PCNA homotrimeric complex to this site. This interacts with pol delta to extend the newly‑formed DNA strand. Human RFC1 is 1148 amino acids (aa) in length. It contains a phosphorylated-DNA binding region (aa 366‑477), a PCNA binding domain (aa 478‑712), an ATPase site (aa 643‑772), and an NLS (aa 1120‑1124). Caspase-3 cleaves RFC-1 into 97, 73 and 65 kDa fragments during apoptosis. There is one potential alternative start site at Met117. Over aa 800‑1093, human RFC1 shares 90% aa identity with mouse RFC1.

Long Name

Replication Factor C 1

Alternate Names

MHCBFB, PO-GA, RECC1, RFC140

Entrez Gene IDs

5981 (Human); 19687 (Mouse); 89809 (Rat)

Gene Symbol

RFC1

UniProt

P35251 (Human)

Additional RFC1 Products

Product Documents for Human RFC1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RFC1 Antibody

For research use only

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