Human Ryk Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB4907N Datasheet / COA / SDS

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FAB4907N-100UG

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Human Ryk Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ryk in ELISA.

Source

Monoclonal Mouse IgG2a Clone # 484804

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ryk
Met1-Arg224
Accession # P34925

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ryk

Ryk (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human Ryk is 604 amino acids (aa) in length. It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human Ryk is 94% aa identical to mouse Ryk.

Long Name

Receptor Type Tyrosine Protein Kinase RYK

Entrez Gene IDs

6259 (Human); 20187 (Mouse); 140585 (Rat)

Alternate Names

D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK