Human S1P3/EDG-3 APC-conjugated Antibody

Catalog #: FAB7524A Datasheet / COA / SDS

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FAB7524A

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Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry.

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Human S1P3/EDG-3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human S1P₃/EDG‑3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 776808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human S1P₃/EDG‑3
Accession # Q99500

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of S1P₃/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P₃/EDG-3 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human S1P₃/EDG-3 and eGFP was stained with either (A) Mouse Anti-Human S1P₃/EDG-3 APC-conjugated Monoclonal Antibody (Catalog # FAB7524A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P₃), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P₃ interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P₃ show 74% aa identity with mouse and rat S1P₃.

Long Name

Endothelial Differentiation Gene 3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Alternate Names

EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3