Human S1P3/EDG-3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human S1P3/EDG‑3 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 776808
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human S1P3/EDG‑3
    Accession # Q99500
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human S1P3/EDG-3 and eGFP was stained with either (A) Mouse Anti-Human S1P3/EDG‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB7524A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P3/EDG-3
Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.
  • Long Name:
    Endothelial Differentiation Gene 3
  • Entrez Gene IDs:
    1903 (Human); 13610 (Mouse); 306792 (Rat)
  • Alternate Names:
    EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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