Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 776808
View all S1P3/EDG-3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P3/EDG‑3
Accession # Q99500

Specificity

Detects human S1P

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human S1P3/EDG-3 APC-conjugated Antibody

Detection of S1P3/EDG-3 antibody in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP antibody by Flow Cytometry.

Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human S1P3/EDG-3 and eGFP was stained with either (A) Mouse Anti-Human S1P3/EDG-3 APC-conjugated Monoclonal Antibody (Catalog # FAB7524A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P3/EDG-3 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NS0 mouse myeloma cell line transfected with human S1P3/EDG-3 and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.

Long Name

Endothelial Differentiation Gene 3

Alternate Names

EDG3, LPB3, S1PR3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Gene Symbol

S1PR3

UniProt

Q99500 (Human)

Additional S1P3/EDG-3 Products

Product Documents for Human S1P3/EDG-3 APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P3/EDG-3 APC-conjugated Antibody

For research use only

Citations for Human S1P3/EDG-3 APC-conjugated Antibody

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