Human S1P3/EDG-3 APC-conjugated Antibody

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Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry.
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Human S1P3/EDG-3 APC-conjugated Antibody Summary

Species Reactivity
Detects human S1P3/EDG‑3 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 776808
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human S1P3/EDG‑3
Accession # Q99500
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<SUB>3</SUB>/EDG-3 antibody in NS0 Mouse Cell Line Transfected with Human S1P<SUB>3</SUB>/EDG-3 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human S1P3/EDG-3 and eGFP was stained with either (A) Mouse Anti-Human S1P3/EDG-3 APC-conjugated Monoclonal Antibody (Catalog # FAB7524A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.

Long Name
Endothelial Differentiation Gene 3
Entrez Gene IDs
1903 (Human); 13610 (Mouse); 306792 (Rat)
Alternate Names
EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3

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