Human SHARPIN Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8100T Datasheet / COA / SDS

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FAB8100T-100UG

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Human SHARPIN Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SHARPIN in ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 881816

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human SHARPIN
Ala27-Ala182
Accession # Q9H0F6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SHARPIN

SHARPIN (Shank-associated RH Domain-interacting Protein), also called SIPL1 (Shank Interacting Protein-like 1) is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFk beta, thus regulating immune and inflammatory responses. SHARPIN is widely expressed, with upregulated expression in multiple tumor types. Human SHARPIN is a 387 amino acid (aa) protein containing self-association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human SHARPIN shares 67% and 66% aa sequence identity with mouse and rat SHARPIN, respectively.

Long Name

SHANK-associated RH Domain Interactor

Entrez Gene IDs

81858 (Human); 106025 (Mouse); 81859 (Rat)

Alternate Names

DKFZp434N1923; hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; SHARPIN; SIPL1; SIPL1DKFZP434N1923