Human SHARPIN Antibody

Catalog # Availability Size / Price Qty
MAB8100
MAB8100-SP
Human SHARPIN Antibody in Western Blot
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Human SHARPIN Antibody Summary

Species Reactivity
Human
Specificity
Detects human SHARPIN in ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 881816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SHARPIN
Ala27-Ala182
Accession # Q9H0F6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SHARPIN by Western Blot. View Larger

Detection of Human SHARPIN by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SHARPIN Monoclonal Antibody (Catalog # MAB8100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SHARPIN at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHARPIN

SHARPIN (Shank-associated RH Domain-interacting Protein), also called SIPL1 (Shank Interacting Protein-like 1) is an essential component of the cytosolic LUBAC ubiquitin conjugation complex. It conjugates linear ubiquitin chains to IKK gamma and activates NFk beta, thus regulating immune and inflammatory responses. SHARPIN is widely expressed, with upregulated expression in multiple tumor types. Human SHARPIN is a 387 amino acid (aa) protein containing self-association, SHANK1 interaction, and zinc finger domains. A 326 aa isoform diverges after aa 308 and lacks the zinc finger domain. Within aa 27-182, human SHARPIN shares 67% and 66% aa sequence identity with mouse and rat SHARPIN, respectively.

Long Name
SHANK-associated RH Domain Interactor
Entrez Gene IDs
81858 (Human); 106025 (Mouse); 81859 (Rat)
Alternate Names
DKFZp434N1923; hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; SHARPIN; SIPL1; SIPL1DKFZP434N1923

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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