Human SHBG Quantikine ELISA Kit

(3 citations)
(1 Review)
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.012 nmol/L
  • Assay Range
    0.1 - 4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human SHBG
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring SHBG.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.5651.362.720.5621.352.71
Standard Deviation0.0320.040.0970.0420.0790.131
CV%5.733.67.65.84.8


Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1.082.55.011.052.524.97
Standard Deviation0.060.120.130.070.150.18
CV%5.64.82.66.363.6

Recovery

The recovery of human SHBG spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 87-96
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SHBG were serially diluted with Calibrator Diluent RD5-24 (1:15) to produce samples with values within the dynamic range of the assay.
Human SHBG Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SHBG
SHBG (Sex Hormone-binding Globulin), also known as ABP (Androgen-binding Protein), is a variably glycosylated, secreted, non-disulfide-linked homodimer. Male and female sex hormones bind SHBG with equal affinity. This binding protein can be found in the circulation where it regulates hormone access to target tissues. Human SHBG shares approximately 67% amino acid sequence identity with mouse and rat SHBG.
    • Long Name
      Sex Hormone-binding Globulin
    • Entrez Gene IDs
      6462 (Human);
    • Alternate Names
      ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    3 Citations: Showing 1 - 3
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    Species
    Sample Type
    1. Analysis of VEGFA Variants and Changes in VEGF Levels Underscores the Contribution of VEGF to Polycystic Ovary Syndrome
      PLoS ONE, 2016;11(11):e0165636.
      Species: Human
      Sample Type: Serum
    2. Alterations of bone density, microstructure, and strength of the distal radius in male patients with rheumatoid arthritis: a case-control study with HR-pQCT.
      Authors: Zhu T, Griffith J, Qin L, Hung V, Fong T, Au S, Li M, Lam Y, Wong C, Kwok A, Leung P, Li E, Tam L
      J Bone Miner Res, 2014;29(9):2118-29.
      Species: Human
      Sample Type: Serum
    3. A randomized phase II presurgical trial of transdermal 4-hydroxytamoxifen gel versus oral tamoxifen in women with ductal carcinoma in situ of the breast.
      Authors: Lee O, Page K, Ivancic D, Helenowski I, Parini V, Sullivan M, Margenthaler J, Chatterton R, Jovanovic B, Dunn B, Heckman-Stoddard B, Foster K, Muzzio M, Shklovskaya J, Skripkauskas S, Kulesza P, Green D, Hansen N, Bethke K, Jeruss J, Bergan R, Khan S
      Clin Cancer Res, 2014;20(14):3672-82.
      Species: Human
      Sample Type: Plasma
    Average Rating: 5 (Based on 1 review)

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     Human SHBG Quantikine ELISA Kit DSHBG0B
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      Anonymous 12/02/2016
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     Human SHBG Quantikine ELISA Kit DSHBG0B
    : Human SHBG Quantikine ELISA Kit [DSHBG0B]

    Summary

    Sample TestedSerum

    FAQs

    1. What is the normal appearance of Assay Diluent RD1-75?

      • Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

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