SHBG (Sex Hormone-binding Globulin), also known as ABP (Androgen-binding Protein), is a variably glycosylated, secreted, non-disulfide-linked homodimer. Male and female sex hormones bind SHBG with equal affinity. This binding protein can be found in the circulation where it regulates hormone access to target tissues. Human SHBG shares approximately 67% amino acid sequence identity with mouse and rat SHBG.
Human SHBG Quantikine ELISA Kit
R&D Systems | Catalog # DSHBG0B
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Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.012 nmol/L
Assay Range
0.063-4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human SHBG Quantikine ELISA Kit
The Quantikine® Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SHBG.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human SHBG.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (nmol/L) | 0.565 | 1.36 | 2.72 | 0.562 | 1.35 | 2.71 |
| Standard Deviation | 0.032 | 0.040 | 0.097 | 0.042 | 0.079 | 0.131 |
| CV% | 5.7 | 3.0 | 3.6 | 7.6 | 5.8 | 4.8 |
Recovery for Human SHBG Quantikine ELISA Kit
The recovery of human SHBG spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 92 | 87-96 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SHBG were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human SHBG Quantikine ELISA Kit
Human SHBG Standard Curve
Human SHBG ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SHBG
Long Name
Sex Hormone-binding Globulin
Alternate Names
ABP
Entrez Gene IDs
6462 (Human)
Gene Symbol
SHBG
Additional SHBG Products
Product Documents for Human SHBG Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SHBG Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human SHBG Quantikine ELISA Kit
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human SHBG Quantikine ELISA Kit
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Q: What is the normal appearance of Assay Diluent RD1-75?
A: Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.