Human SIRP alpha /CD172a Fluorescein-conjugated Antibody

Catalog #: FAB4546F Datasheet / COA / SDS

Discontinued Product

FAB4546F has been discontinued.
View all SIRP alpha/CD172a products.
Detection of SIRP alpha /CD172a in U937 Human Cell Line by Flow Cytometry.
1 Image
Product Details

Human SIRP alpha /CD172a Fluorescein-conjugated Antibody Summary

Species Reactivity
Detects human SIRP alpha /CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed.
Monoclonal Mouse IgG2B Clone # 602411
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha /CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SIRPa/CD172a antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SIRP alpha /CD172a in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4546F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SIRP alpha/CD172a

SIRP alpha (Signal Regulatory Protein alpha), also known as CD172a, SHPS1 and BIT is a variably glycosylated 90-120 kDa member of the SIRP family of proteins.  It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts.  SIRP alpha has a variety of functions, including presynaptic organization, inhibition of integrin action, and induction of myogenesis.  It binds to CD47 and likely other ligands.  Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein.  It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347).  Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases.  There is one alternative start site at Met102 plus a four aa insertion after Gln421.  Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.

Long Name
Signal-regulatory Protein alpha
Entrez Gene IDs
140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)
Alternate Names
BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human SIRP alpha /CD172a Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human SIRP alpha /CD172a Fluorescein-conjugated Antibody and earn rewards!

Have you used Human SIRP alpha /CD172a Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review