Human Sirtuin 3/SIRT3 Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # AF7488AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Sheep IgG
View all Sirtuin 3/SIRT3 Primary Antibodies »

Product Specifications

Specificity

Detects human Situin 3/SIRT3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SIRT2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Sirtuin 3/SIRT3 Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sirtuin 3/SIRT3

SIRT3 (SIR2-like protein 3) is a 28-30 kDa, NAD+-dependent class III member of the sirtuin protein family. It is widely expressed, and found apparently in both the nucleus, where it deacetylates histones, and in the mitochondrial matrix, where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates, including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100 aa, followed by a sertuin-type deacetylase domain (aa 126-382) that contains an NAD binding segment (aa 145-165). Although uncertainty exists relative to its intracellular location, it would appear that SIRT3 is initially synthesized as a 42-44 kDa "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28-30 kDa short form. There is one potential alternative start site at Met143. Over aa 101-399, human and mouse SIRT3 share 84% aa sequence identity.

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Entrez Gene IDs

23410 (Human); 64384 (Mouse); 293615 (Rat)

Gene Symbol

SIRT3

UniProt

Q9NTG7 (Human)

Additional Sirtuin 3/SIRT3 Products

Product Documents for Human Sirtuin 3/SIRT3 Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Sirtuin 3/SIRT3 Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Epigenetics

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