Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Sirtuin 3/SIRT3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Sirtuin 3/SIRT3
Ser101-Lys399
Accession # Q9NTG7

Specificity

Detects human Situin 3/SIRT3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SIRT2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Sirtuin 3/SIRT3 Antibody

Detection of Human Sirtuin 3/SIRT3 antibody by Western Blot.

Detection of Human Sirtuin 3/SIRT3 by Western Blot.

Western blot shows lysates of human liver tissue, human heart ventricle tissue, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 3/SIRT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7488) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sirtuin 3/SIRT3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Sirtuin 3/SIRT3 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Human liver tissue, human heart ventricle tissue, and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sirtuin 3/SIRT3

SIRT3 (SIR2-like protein 3) is a 28-30 kDa, NAD+-dependent class III member of the sirtuin protein family. It is widely expressed, and found apparently in both the nucleus, where it deacetylates histones, and in the mitochondrial matrix, where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates, including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100 aa, followed by a sertuin-type deacetylase domain (aa 126-382) that contains an NAD binding segment (aa 145-165). Although uncertainty exists relative to its intracellular location, it would appear that SIRT3 is initially synthesized as a 42-44 kDa "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28-30 kDa short form. There is one potential alternative start site at Met143. Over aa 101-399, human and mouse SIRT3 share 84% aa sequence identity.

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Entrez Gene IDs

23410 (Human); 64384 (Mouse); 293615 (Rat)

Gene Symbol

SIRT3

UniProt

Q9NTG7 (Human)

Additional Sirtuin 3/SIRT3 Products

Product Documents for Human Sirtuin 3/SIRT3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Sirtuin 3/SIRT3 Antibody

For research use only

Related Research Areas

Epigenetics

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