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Human Somatostatin R2/SSTR2 Alexa Fluor® 647-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of Somatostatin R2/SSTR2 in HEK293 Human Cell Line Transfected with Human Somatostatin R2/SSTR2 and eGFP by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects human Somatostatin R2/SSTR2. Stains SSTR2 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2A Clone # 402038
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with Somatostatin R2/SSTR2
    Met1-Ile369
    Accession # P30874
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry Somatostatin R2/SSTR2 Antibody (402038) [Alexa Fluor® 647]
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Detection of Somatostatin R2/SSTR2 in HEK293 Human Cell Line Transfected with Human Somatostatin R2/SSTR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Somatostatin R2/SSTR2 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human Somatostatin R2/SSTR2 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC4224R). Quadrant markers were set based on isotype control antibody (Catalog # IC003R).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Somatostatin R2/SSTR2

Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.

  • Long Name:
    Somatostatin Receptor Type 2
  • Entrez Gene IDs:
    6752 (Human); 20606 (Mouse); 54305 (Rat)
  • Alternate Names:
    Smstr2; Somatostatin R2; somatostatin receptor 2; somatostatin receptor type 2; SomatostatinR2; SRIF-1; SS2R; SS-2-R; SS2-R; SST2; SSTR2
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